L leucine

Manufactured by J&K Scientific

Sourced in China

L-Leucine is an essential amino acid. It is a white crystalline powder that is soluble in water and alcohol. L-Leucine is a branch-chained amino acid that plays a role in protein synthesis.

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Lab products found in correlation

2 protocols using l leucine

Metabolite Extraction and Derivatization

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HPLC-grade
methanol was purchased
from Merck (Darmstadt, Germany). Tridecanoic acid (internal standard) N-methyl-N-(trimethylsilyl)-trifluoroacetamide
(MSTFA), methoxyamine hydrochloride, pyridine, dichloromethane, linoleic
acid, glycerol-3-phosphate, 4-hydroxyproline, hypoxanthine, and l-aspartate were all supplied by Sigma-Aldrich (St. Louis, MO). l-Leucine and l-glutamate were provided by J&K
Scientific Ltd. (Beijing, China). 2-Ketoisocaproic acid and l-glutamine were purchased from AccuStandard (New Haven, CT) and LookChem
Chemicals (Shanghai, China), respectively.

Zhang S., Lu X., Hu C., Li Y., Yang H., Yan H., Fan J., Xu G., Abnet C.C, & Qiao Y. (2020). Serum Metabolomics for Biomarker Screening of Esophageal Squamous Cell Carcinoma and Esophageal Squamous Dysplasia Using Gas Chromatography-Mass Spectrometry. ACS Omega, 5(41), 26402-26412.

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Bacillus cereus Leucine Dehydrogenase Purification

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Leucine dehydrogenase (LDH) from Bacillus cereus was in our laboratory. Plasmid pET-28a (+) and Escherichia coli BL21 (DE3) were procured from Novagen (Nanjing, China) and used as the gene expression vector and host, respectively. Kanamycin, isopropyl-β-D-thiogalactopyranoside (IPTG), 1-fluoro-2,4-dinitrophenyl-5-L-alanineamide (FDAA), NAD⁺, and NADH were obtained from TCI (Shanghai, China). α-keto acids (4-methyl-2-oxopentanoic acid, 3-methyl-2-oxobutanoic acid, 2-oxobutanoic acid, 3,3-dimethyl-2-oxobutanoic acid, 2-oxopropanoic acid, 2-oxo-3-phenylpropanoic acid) and α-amino acids (L-leucine, L-valine, L-2-aminobutyric acid, L-tert-leucine, L-alanine, L-phenylalanine) were purchased from J&K Chemical (Beijing, China). All other chemicals were purchased from commercial sources, at least reagent grade, and were used without further purification.

Mu X., Feng X., Wu T., Zhou F., Nie Y, & Xu Y. (2021). Transamination-Like Reaction Catalyzed by Leucine Dehydrogenase for Efficient Co-Synthesis of α-Amino Acids and α-Keto Acids. Molecules, 26(23), 7287.

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