8 well chamber slide

Manufactured by EQT

Sourced in United States, United Kingdom

The 8-well chamber slide is a laboratory equipment designed to provide a controlled environment for cell culture and microscopic observation. It features eight individual chambers, allowing for multiple samples to be analyzed simultaneously. The slide is made of high-quality materials and is suitable for a variety of cell types and experimental setups.

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Lab products found in correlation

Lsm 700, by Zeiss (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (3 mentions) B 5 1 2, by Merck Group (3 mentions) Fitc conjugated antibody to mouse igg, by Merck Group (3 mentions) Zen black edition software, by Zeiss (2 mentions) Zen software, by Zeiss (2 mentions) Lsm710 live duo confocal microscope, by Zeiss (1 mentions) D9542, by Merck Group (1 mentions) Vectashield h 1000, by Vector Laboratories (1 mentions) P1951, by Merck Group (1 mentions) Alexa fluor 549 conjugated antibodyto rabbit igg, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Anti itgb1, by Cell Signaling Technology (1 mentions) Anti itgb3, by Cell Signaling Technology (1 mentions) Anti itgav, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugated goat anti mouse igg or anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Anti vim, by Abcam (1 mentions) Anti cd47, by Abcam (1 mentions) Alexa fluor 488 or 594, by Thermo Fisher Scientific (1 mentions)

7 protocols using 8 well chamber slide

Microscopic Imaging of α-Tubulin in MDA-MB-231 Cells

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MDA-MB-231 cells (2.4 × 10⁴ cells/well) were grown on an 8-well chamber slide (Lab-Tech, Waltham, USA) for 24 h prior to treatment with reagents. Cells were treated for 24 h with compound 5 or DMSO as a control for 24 h. Cells were fixed in 4% paraformaldehyde in PBS and permeabilized with 0.5% Triton X-100 in PBS. Fixed cells were labeled with mouse monoclonal antibody to α-tubulin (B5-1-2, Sigma, St. Louis, MO, USA) followed by FITC-conjugated antibody to mouse IgG (Sigma) [31 (link)]. Nuclei were labeled with DAPI (Sigma). Fluorescence labeled cells were observed using confocal microscope (LSM700, Zeiss, White Plains, NY, USA) controlled by ZEN (black edition) software (Zeiss). Confocal images were stacked and merged using ZEN (black edition) software. Final images were prepared using Adobe Photoshop.

Saito Y., Mizokami A., Izumi K., Naito R., Goto M, & Nakagawa-Goto K. (2021). α-Trifluoromethyl Chalcones as Potent Anticancer Agents for Androgen Receptor-Independent Prostate Cancer. Molecules, 26(9), 2812.

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Enteroid Cell Ablation Imaging

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Enteroids were plated on an 8-well chamber slide (Lab-tech) 2 days before the imaging experiments. Image acquisition was performed with an LSM710 LIVE Duo confocal microscope (Zeiss). Single-cell ablations of enteroid cells were performed through LSM510 two-photon microscope (Zeiss). The details of the procedures are given in the Supplemental Experimental Procedures.

Matsu-ura T., Dovzhenok A., Aihara E., Rood J., Le H., Ren Y., Rosselot A.E., Zhang T., Lee C., Obrietan K., Montrose M.H., Lim S., Moore S.R, & Hong C.I. (2016). Intercellular Coupling of the Cell Cycle and Circadian Clock in Adult Stem Cell Culture. Molecular cell, 64(5), 900-912.

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Visualizing Microtubule Dynamics in MDA-MB-231 Cells

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MDA-MB-231 cells were seeded in the 8-well chamber slide (Lab-Tech) 24 h prior to treatment with compounds. After 24 h treatment, cells were fixed with 4% paraformaldehyde in PBS followed by permeabilization with 0.5% Triton-X100. Cells were then probed with antibody to α-tubulin (B5–1-2, Sigma) followed by FITC-conjugated antibody to mouse IgG (Sigma). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma). Microtubules were detected using a confocal microscope (Zeiss, LSM700) controlled by ZEN software (Zeiss). Final images were reconstructed from 12−18 sections acquired at 0.6−1 μm intervals and merged using ZEN (black edition) software. Experiments were repeated at least twice for each compound at each concentration. Final images were prepared using Adobe Photoshop CS6.

Aimaiti S., Suzuki A., Saito Y., Fukuyoshi S., Goto M., Miyake K., Newman D.J., O’Keefe B.R., Lee K.H, & Nakagawa-Goto K. (2018). Corymbulosins I−W, Cytotoxic Clerodane Diterpenes from the Bark of Laetia corymbulosa. The Journal of organic chemistry, 83(2), 951-963.

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Immunofluorescent Staining of Cytoskeletal Structures

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KB-VIN cells were seeded in an 8-well chamber slide (Lab-Tech) at a density of 1 × 10⁵ per mL and incubated overnight. After 24 h of treatment with tested compounds, the cells were fixed with 4% paraformaldehyde in PBS for 10 min followed by permeabilization with 0.5% Triton X-100 in PBS for 5 min at room temperature. Cells were then blocked with 5% normal goat serum in 1% BSA in washing buffer (0.01% Tween 20 in PBS) for 30 min. Cells were labeled with monoclonal antibody to α-tubulin (T5168, clone B-5–1-2, Sigma-Aldrich) for 2 h followed by fluorescein isothiocyanate (FITC)-labeled anti-mouse IgG secondary antibody (F5262, Sigma-Aldrich) for 1 h. F-actin was stained with tetramethylrhodamine (TRITC)-labeled phalloidin (P1951, Sigma-Aldrich) for 30 min while DNA was stained with 4’,6-diamidino-2-phenylindole (DAPI) (D9542, Sigma-Aldrich) for 5 min. Stained cells were mounted with Vectashield (H-1000, Vector Laboratories). The images were captured by confocal fluorescence microscope (Zeiss LSM700) (Laser lines: 405 nm for DAPI, 488 nm for FITC, and 555 nm for TRITC) operated by ZEN software (Zeiss). Final images were analyzed by ZEN light and processed with Photoshop 7 (Adobe).

Teng Y.N., Wang Y., Hsu P.L., Xin G., Zhang Y., Morris-Natschke S.L., Goto M, & Lee K.H. (2018). Mechanism of Action of Cytotoxic Compounds from the Seeds of Euphorbia lathyris. Phytomedicine : international journal of phytotherapy and phytopharmacology, 41, 62-66.

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Immunocytochemical Analysis of Microtubule and Mitotic Markers

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Immunocytochemical analysis was performed as previously described [26 (link)]. KB-VIN cells were grown on an 8-well chamber slide (Lab-Tech) for 24 h prior to treatment with the compound at a concentration of three-fold IC₅₀. CA-4 was used at 0.2 μM (Figure 2A and Supporting Information, Supplementary Figure S1). After treatment of cells with the agent for 24 h, cells were fixed with 4% paraformaldehyde in PBS and permeabilized with 0.5% Triton X-100 in PBS. Fixed cells were labeled with mouse monoclonal antibody to α-tubulin (B5-1-2, Sigma) and rabbit IgG to Ser10-phosphorylated histone H3 (p-H3) (#06570, EMD Millipore), followed by FITC-conjugated antibody to mouse IgG (Sigma) and Alexa Fluor 549-conjugated antibody to rabbit IgG (Life Technologies). Nuclei were labeled with DAPI (Sigma). Fluorescently-labeled cells were observed using a confocal microscope (Zeiss, LSM700) with ZEN (black edition) software (Zeiss). The 15~20 optical sections acquired at 0.5~1 µm intervals were stacked and reconstructed using ZEN (black edition) software. Experiments were repeated at least twice for each compound. Final images were prepared using Adobe Photoshop CS3.

Zhang Y., Goto M., Oda A., Hsu P.L., Guo L.L., Fu Y.H., Morris-Natschke S.L., Hamel E., Lee K.H, & Hao X.J. (2019). Antiproliferative Aspidosperma-Type Monoterpenoid Indole Alkaloids from Bousigonia mekongensis Inhibit Tubulin Polymerization. Molecules, 24(7), 1256.

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Immunofluorescence Analysis of Cell Adhesion Molecules

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Cells (1 × 10⁴/well) were seeded on 8-well chamber slides (Lab-Tech) after transfection. The cells were fixed, permeated and blocked. Then, they were incubated with the following antibodies: anti-VIM (1:200, Abcam), anti-ITGAV (1:1000, Cell Signaling Technology), anti-ITGB1 (1:1000, Cell Signaling Technology), anti-ITGB3 (1:500, Cell Signaling Technology) or anti-CD47 (1:200, Abcam). The secondary antibody, Alexa Fluor 488-conjugated goat anti-mouse IgG or anti-rabbit IgG (1:500, Invitrogen), was applied for 1 hour. Slides were mounted with antifading solution containing 4′-6′-diamidino-2-phenyl-indole (Vector Laboratories). Images were taken using a confocal microscope (Zeiss). All experiments were conducted in triplicate.

Yang S.Y., Choi S.A., Lee J.Y., Park A.K., Wang K.C., Phi J.H., Koh E.J., Park W.Y., Park S.H., Hwang D.W., Jung H.W, & Kim S.K. (2015). miR-192 suppresses leptomeningeal dissemination of medulloblastoma by modulating cell proliferation and anchoring through the regulation of DHFR, integrins, and CD47. Oncotarget, 6(41), 43712-43730.

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Immunofluorescence Assay for CA9, Vimentin, and Keratin

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The expression levels of carbonic anhydrase 9 (CA9), vimentin, and pan-keratin were compared by using immunofluorescence. Cancer cells and normal cells from patients were cultured in 8-well chamber slides (Labtech, East Sussex, UK). Each cell was seeded for 5 hours after trypsin treatment and removal of Matrigel. Cells were fixed with 4% paraformaldehyde solution for 10 minutes and then washed with PBS. Cells were then permeated in 0.5% Triton X-100 in PBS for 5 minutes, blocked in 5% bovine serum albumin (BSA) in PBS for 20 minutes, and incubated with diluted primary antibodies to CA9 (1:100; Novus Biologicals, Littleton, CO, USA), vimentin, and pan-keratin (Cell Signaling Technology, Danvers, MA, USA) in 5% BSA at 4℃ for 16 hours. Cells were washed with PBS and then incubated with secondary antibodies tagged with Alexa Fluor 488 or 594 (Molecular Probes, Eugene, OR, USA) at 25℃ for 1 hour (1:200). Images were acquired by laser scanning confocal microscopy (laser scanning microscope [LSM] Meta 700; Carl Zeiss, Oberkochen, Germany) and analyzed with LSM Image Browser software.

Na J.C., Kim J.H., Kim S.Y., Gu Y.R., Jun D.Y., Lee H.H., Yoon Y.E., Choi K.H., Hong S.J, & Han W.K. (2020). Establishment of patient-derived three-dimensional organoid culture in renal cell carcinoma. Investigative and Clinical Urology, 61(2), 216-223.

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