Implantation sites, harvested as above, were dehydrated in 20% sucrose overnight at 4°C. flash frozen in optimal cutting temperature (Sakura USA, Torrance, CA) with 2-methylbutane, and cooled with dry ice. Cassettes were stored at −80°C. Frozen sections (7 μm) were prepared on a Leica CryoStat. For granulocyte-differentiation antigen-1 (Gr-1) staining, frozen slides were fixed in pre-chilled acetone at room temperature (RT). Endogenous peroxidase was quenched with 0.3% H2O2 (Sigma-Aldrich) in methanol (Fisher Scientific, St. Louis, MO). Blocking was performed in PBS, 1% BSA, 5% mouse serum and 5% goat serum. RB6-8C5 (BioXCell 3.5 mg/ml, a rat monoclonal anti-mouse Gr-1 Ab) was used at a dilution of 1:500 in the blocking buffer. Another anti-Gr-1 Ab (1A8) was used at 1:500 (BioXCell). The secondary Ab was a goat anti-rat light chain horse radish peroxidase (HRP) (Jackson Immunoresearch). Staining was visualized with DAB (3, 3′-diaminobenzidine; Vector, IMPACT DAB KIT).
Anti gr 1 ab 1a8
Manufactured by BioXCell
Anti-Gr-1 Ab (1A8) is a rat monoclonal antibody that recognizes the Gr-1 antigen, which is expressed on the surface of granulocytes, including neutrophils, eosinophils, and monocytes. This antibody can be used for the identification and characterization of Gr-1-positive cells in various applications.
Automatically generated - may contain errors
2 protocols using anti gr 1 ab 1a8
1
Gr-1 Immunohistochemistry Protocol
2
Granulocyte Differentiation Antigen-1 Staining
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Implantation sites, harvested as above, were dehydrated in 20% sucrose overnight at 4°C, flash frozen in optimal cutting temperature (Sakura USA, Torrance, CA) with 2‐methylbutane, and cooled with dry ice. Cassettes were stored at −80°C. Frozen sections (7 μm) were prepared on a Leica CryoStat. For granulocyte‐differentiation antigen‐1 (Gr‐1) staining, frozen slides were fixed in pre‐chilled acetone at room temperature (RT). Endogenous peroxidase was quenched with 0.3% H2O2 (Sigma‐Aldrich) in methanol (Fisher Scientific, St. Louis, MO). Blocking was performed in PBS, 1% BSA, 5% mouse serum, and 5% goat serum. RB6‐8C5 (BioXCell 3.5 mg/mL, a rat monoclonal anti‐mouse Gr‐1 Ab) was used at a dilution of 1:500 in the blocking buffer. Another anti‐Gr‐1 Ab (1A8) was used at 1:500 (BioXCell). The secondary Ab was a goat anti‐rat light chain horse radish peroxidase (HRP; Jackson Immunoresearch). Staining was visualized with DAB (3, 3′‐diaminobenzidine; Vector, IMPACT DAB KIT).
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