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3 protocols using ginsenoside rb2

1

Quantification of Ginsenoside Compounds

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Notoginsenoside R1 (R1), ginsenoside Rb1 (Rb1), ginsenoside Rb2 (Rb2), ginsenoside Rb3 (Rb3), ginsenoside Rg1 (Rg1), ginsenoside Rg2 (Rg2), ginsenoside Rf (Rf), ginsenoside Ro (Ro), ginsenoside Rd (Rd), ginsenoside Re (Re), (20S)-ginsenoside Rg3 ((20S)-Rg3), (20R)-ginsenoside Rg3 ((20R)-Rg3), ginsenoside Rk2 (Rk2) (≥98% pure) were provided by the Shanghai Yuanye Biotechnology Co., ltd. (Shanghai, China). P. ginseng, A. macrocephala, P. cocos and G. uralensis applied in the study were purchased from Fusong Shenyuan Changbaishan Ginseng Technology Co., ltd. (Fusong, China).
Acetonitrile was purchased from Fisher Scientific (Waltham, MA, USA). Water was purified through a Milli-Q water purification system (Millipore, MA, USA). TNF-α, IL-1β, IL-6 and NO ELISA kits were provided by Jiangsu Mai Sha Industry Co., ltd (Yancheng, China). Dextran sulfate sodium (DSS) was obtained from MP Biomedicals (California, USA). Cell Counting Kit-8 (CCK-8) was obtained from Biosharp Life Science (Shanghai, China). Lipopolysaccaride (LPS) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Griess reagents were purchased from Promega (Madison, USA).
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2

Ginsenoside and Schisandrol Quantification

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Authentic standards of ginsenoside Rg1, ginsenoside Re, ginsenoside Rf, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rg2, ginsenoside Rh1, ginsenoside Ro, ginsenoside Rb2 and ginsenoside Rd, schisandrol A, schisandrol B were all purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). d-Fructose was purchased from National Institutes for Food and Drug Control (Beijing, China). The purity of each chemical was equal to or greater than 97%. They were stored at 4 °C before use. P. ginseng, S. chinensis, and O. japonicus extract intermediates and SMI samples were from SZYY Group Pharmaceutical Limited (Nanjing, China).
All solvents, including methanol and acetonitrile with a purity of 98% were of chromatographic grade purchased from Fisher (Hampton, NH, USA), all other reagents and chemicals were of analytical grade.
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3

Ginsenoside Compounds Analysis in Danhong Injection

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Ginsenoside Re, ginsenoside Rb 1 , pseudoginsenoside F 11 , ginsenoside Rb 2 , ginsenoside Rb 3 , ginsenoside Rd, and ginsenoside F 2 were purchased from Shanghai Yuanye Biotechnology Co., Ltd. MS-grade water and acetonitrile were acquired from Watsons Ltd and Tedia Company Inc., respectively. All other chemicals used were analytical grade. Danhong injection (DHI, lot: 16011017) ▶ Fig. 5 KEGG analysis revealed the top 20 pathway terms associated with the targets from PPI network.
was purchased from Danhong Pharmaceutical Co., Ltd. Human umbilical vein endothelial cells (HUVEC) were acquired from the American Type Culture Collection (ATCC).
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