Hgt agarose

Manufactured by Lonza

Sourced in United States

2% HGT agarose is a high-gel-strength agarose used for DNA electrophoresis. It provides a stable, uniform matrix for the separation and resolution of nucleic acid fragments.

Automatically generated - may contain errors

Lab products found in correlation

Immobilon p, by Merck Group (2 mentions) Western lightning ecl, by PerkinElmer (2 mentions) Rabbit anti human vwf primary antibody, by Agilent Technologies (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Irdye 800 conjugated goat anti rabbit igg, by LI COR (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Irdye 680 conjugated goat anti mouse igg, by LI COR (1 mentions)

Spelling variants of this product

Agarose (75 citations) SeaKem HGT (P) agarose (2 citations)

2 protocols using hgt agarose

VWF Multimer Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

The equivalent of 1 mU VWF (determined via ELISA) from patient plasma was run in each lane of a 2% HGT agarose (Lonza, Rockland, ME, USA) resolving gel containing 1% sodium dodecyl sulfate (SDS) for 16 hours at 40V followed by electroblot transfer for one hour at 100V to an Immobilon‐P (Millipore, Billerica, MA, USA) membrane in a 4°C buffer containing 25 mmol L⁻¹ Tris, 200 mmol L⁻¹ glycine, 20% methanol and 0.03% SDS. The membrane was blocked for 1 hour with 5% nonfat dry milk, then incubated with an rabbit‐anti‐human VWF primary antibody (DAKO, Carpinteria, CA, USA) for one hour followed by a one‐hour incubation with a HRP‐linked goat anti‐rabbit antibody. Membranes were then developed with Western Lightning‐ECL (Perkin Elmer, Waltham, MA, USA) and bands were visualized by exposure to Fujifilm Super RX (Edison, NJ, USA). Multimer densitometric analysis was done using ImageJ/FIJI (see supplemental methods).

Ng C.J., McCrae K.R., Ashworth K., Sosa L.J., Betapudi V., Manco‐Johnson M.J., Liu A., Dong J., Chung D., White‐Adams T.C., López J.A, & Di Paola J. (2018). Effects of anti‐β2GPI antibodies on VWF release from human umbilical vein endothelial cells and ADAMTS13 activity. Research and Practice in Thrombosis and Haemostasis, 2(2), 380-389.

+ Open protocol

+ Expand

Western Blot Analysis of rVWF

Check if the same lab product or an alternative is used in the 5 most similar protocols

The equivalent of 1 mU rVWF from HEK293T conditioned medium was run in each lane of a 2% HGT agarose (Lonza, Rockland, ME) resolving gel containing 1% sodium dodecyl sulfate (SDS) for 16 hours at 40V followed by electroblot transfer for 1 hour at 100V to an Immobilon-P (Millipore, Billerica, MA) membrane in a 4˚C buffer containing 25mM Tris, 200 mM glycine, 20% methanol and 0.03% SDS. Following a 1 hour block with 5% nonfat dry milk, membranes were incubated with an HRP-conjugated rabbit-anti-human VWF pAb (DAKO, Carpinteria, CA) for 1hr. Membranes were then developed with Western Lightning-ECL (Perkin Elmer, Waltham, MA) and bands were visualized by exposure to Fujifilm Super RX (Edison, NJ).
For two-color analysis, membranes were blocked with Odyssey Blocking Buffer (LI-COR, Lincoln, NE), incubated with AVW-5 and rabbit-anti–c-Myc (Affinity BioReagents, Golden, CO), followed by incubation with IRDye 680–conjugated goat anti–mouse IgG and IRDye 800–conjugated goat anti–rabbit IgG (LI-COR). Membranes were then visualized with the Odyssey infrared imaging system (LI-COR) at BloodCenter of Wisconsin.

White-Adams T.C., Ng C.J., Jacobi P.M., Haberichter S.L, & Di Paola J.A. (2016). Mutations in the D’D3 region of VWF traditionally associated with type 1 VWD lead to quantitative and qualitative deficiencies of VWF. Thrombosis research, 145, 112-118.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!