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Goat f ab 2 anti human igd fitc

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Goat F(ab')2 Anti-Human IgD- FITC is a secondary antibody conjugated with the fluorescent dye FITC (fluorescein isothiocyanate). It is designed to detect and bind to the IgD antibody in human samples.

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2 protocols using goat f ab 2 anti human igd fitc

1

Flow Cytometric Analysis of Human B Cell Subsets

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Mononuclear cells were isolated from peripheral blood using ficoll density gradient centrifugation and stained with the following anti-human antibody staining reagents: Goat F(ab')2 Anti-Human IgD- FITC (Invitrogen) or -APC (clone: IgD26, Miltenyi Biotec); Goat F(ab')2 Anti-Human IgM-PE-Cy5; CD3-Pacific Orange (clone: UCHT1), CD14-Pacific Orange (clone: TK4); CD24-PE-A610 (clone: SN3; Invitrogen); CD19-APC-Cy7 (clone: SJ25C1); CD38-Pacific Blue (clone: HB7); CD23-PE-Cy7 clone: M-L233); CD21-PE-Cy5 (clone B-ly4); CD27-PE (clone: L128, BD Pharmingen); CD138-APC (clone: 44F9, Miltenyi Biotec) and mitotracker green. Approximately 0.1–3 × 105 cells were collected for each population using a BD FACS Aria II (BD Biosciences).
Tipton C.M., Fucile C.F., Darce J., Chida A., Ichikawa T., Gregoretti I., Schieferl S., Hom J., Jenks S., Feldman R.J., Mehr R., Wei C., Lee F.E., Cheung W.C., Rosenberg A.F, & Sanz I. (2015). Diversity, cellular origin and autoreactivity of antibody-secreting cell expansions in acute Systemic Lupus Erythematosus. Nature immunology, 16(7), 755-765.
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2

Flow Cytometric Analysis of Human B Cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mononuclear cells were isolated from peripheral blood using ficoll density gradient centrifugation and stained with the following anti-human antibody staining reagents: Goat F(ab')2 Anti-Human IgD- FITC (Invitrogen) or -APC (clone: IgD26, Miltenyi Biotec); Goat F(ab')2 Anti-Human IgM-PE-Cy5; CD3-Pacific Orange (clone: UCHT1), CD14-Pacific Orange (clone: TK4); CD24-PE-A610 (clone: SN3; Invitrogen); CD19-APC-Cy7 (clone: SJ25C1); CD38-Pacific Blue (clone: HB7); CD23-PE-Cy7 clone: M-L233); CD21-PE-Cy5 (clone B-ly4); CD27-PE (clone: L128, BD Pharmingen); CD138-APC (clone: 44F9, Miltenyi Biotec) and mitotracker green. Approximately 0.1–3 × 105 cells were collected for each population using a BD FACS Aria II (BD Biosciences).
Tipton C.M., Fucile C.F., Darce J., Chida A., Ichikawa T., Gregoretti I., Schieferl S., Hom J., Jenks S., Feldman R.J., Mehr R., Wei C., Lee F.E., Cheung W.C., Rosenberg A.F, & Sanz I. (2015). Diversity, cellular origin and autoreactivity of antibody-secreting cell expansions in acute Systemic Lupus Erythematosus. Nature immunology, 16(7), 755-765.
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