Anti polymerase 2 antibody

Manufactured by Active Motif

The Anti-polymerase II antibody is a laboratory tool used to detect and study the presence and distribution of RNA polymerase II, a critical enzyme involved in the transcription of genetic information from DNA to RNA. This antibody provides a reliable and specific means of identifying and analyzing the localization and expression of RNA polymerase II in various experimental systems.

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Lab products found in correlation

Dpnii restriction enzyme, by New England Biolabs (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions) Nextseq 500, by Illumina (1 mentions) Ab2743, by Abcam (1 mentions) Anti dnmt3a, by Abcam (1 mentions) Anti dnmt3b, by Abcam (1 mentions) Anti cbp, by Santa Cruz Biotechnology (1 mentions) Anti dnmt1, by Abcam (1 mentions) Anti dimethyl histone h3 lys9, by Abcam (1 mentions) Anti hdac4, by Abcam (1 mentions) Anti hdac5, by Abcam (1 mentions)

2 protocols using anti polymerase 2 antibody

Hi-ChIP Assay for Plant Tissues

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For Hi-ChIP experiments, the nuclei were isolated from the shoots and roots using the same procedure as in the in situ Hi-C experiments, and the Hi-ChIP protocol from Mumbach et al. 2016 was then applied using the DpnII restriction enzyme (New England Biolabs) and the anti-polymerase II antibody (Active Motif, 39097). The quality of the libraries was assessed with Agilent 2100 Bioanalyzer (Agilent), and the libraries were subjected to 2 × 75 bp paired-end high-throughput sequencing by NextSeq 500 (Illumina).

Concia L., Veluchamy A., Ramirez-Prado J.S., Martin-Ramirez A., Huang Y., Perez M., Domenichini S., Rodriguez Granados N.Y., Kim S., Blein T., Duncan S., Pichot C., Manza-Mianza D., Juery C., Paux E., Moore G., Hirt H., Bergounioux C., Crespi M., Mahfouz M.M., Bendahmane A., Liu C., Hall A., Raynaud C., Latrasse D, & Benhamed M. (2020). Wheat chromatin architecture is organized in genome territories and transcription factories. Genome Biology, 21, 104.

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Oligonucleotide and Antibody Preparation

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Oligonucleotides - Synthetic oligonucleotides (Retrogen Inc., Carlsbad, CA; Integrated DNA Tech. Inc., San Diego, CA) were used in these experiments. Double-stranded oligonucleotides were generated by annealing synthetic oligonucleotides with respective complementary sequence. Antibodies - Rabbit polyclonal anti-HP1β (D-15): sc-10217, anti-HDAC4, anti-HDAC5, anti-Dnmt1, anti-Dnmt3a, anti-Dnmt3b, anti-dimethyl-histone H3 (Lys9), anti-thyroid hormone receptor (alpha 1+2) (ab1131) and monoclonal anti-thyroid hormone receptor (alpha 1 and beta 1) (ab2743), anti-H2A.Z and anti-SRC1 antibodies were purchased from Abcam Inc. (Cambridge, MA) and anti-CBP was from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-acetyl-histone H3, anti-acetyl-histone H4, anti-acetyl-histone H4 (Lys5), anti-acetyl-histone H4 (Lys8), anti-acetyl-histone H4 (Lys16), anti-acetyl-histone H4 (Lys12) and anti-MeCP2 were purchased from Upstate Biotechnology (Lake Placid, NY). Anti-polymerase II antibody was from Active Motif (Carlsbad, CA). Horseradish peroxidase linked anti-rabbit and anti-mouse IgGs were from Amersham Biosciences (Piscataway, NJ).

Raychaudhuri N., Thamotharan S., Srinivasan M., Mahmood S., Patel M.S, & Devaskar S.U. (2014). Postnatal Exposure to a High Carbohydrate Diet Interferes Epigenetically with Thyroid Hormone Receptor Induction of the Adult Male Rat Skeletal Muscle Glucose Transporter Isoform 4 Expression. The Journal of nutritional biochemistry, 25(10), 1066-1076.

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