Cfsp22300

The materials consisted of nitrocellulose (FF120HP, Whatman, Cytiva, Marlborough, MA, USA), cellulose (CFSP22300, EMD Millipore, St. Louis, MO, USA), glass fiber (Ahlstrom, 8951 chopped glass with binder), and polymeric laminates with an adhesive (Tekra Corporation, New Berlin, WI, USA). Manufacturer-reported specifications for FF120HP include a thickness of 100 μm (on a 100 μm polyester backing) and a capillary rise time of 90 to 150 s per 4 cm. Further, the porosity of the membrane was measured to be ~70% [19 ]. A CO₂ laser system (Universal, VLS 3.5, Scottsdale, AZ, USA) was used to cut the materials to size. Capture reagents were applied onto the nitrocellulose either via hand-spotting using a small-volume pipette or striping using a liquid dispensing system (AD3320, BioDot, Irvine, CA, USA). Nitrocellulose strips were placed on 10 mil thick polyester with an adhesive base and then the cellulose pad was placed downstream and overlapped the nitrocellulose by 2 mm. A layer of 10 mil thick polyester was placed over the cellulose pads to facilitate contact between the cellulose pads and the nitrocellulose strips, and spacers were utilized to support uniform contact across the multiple strips in a device. Glass fiber pads were attached to the upstream end of the nitrocellulose strips for any devices involving target nucleic acid hybridization.