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Rhodamine red x goat anti mouse igg r6393

Manufactured by Thermo Fisher Scientific
Sourced in United States

Rhodamine Red‐X goat anti‐mouse IgG (R6393) is a fluorescent secondary antibody. It is designed to detect and bind to mouse immunoglobulin G (IgG) antibodies. The antibody is conjugated with the Rhodamine Red‐X dye, which fluoresces in the red spectrum and can be used for fluorescence-based detection and visualization applications.

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2 protocols using rhodamine red x goat anti mouse igg r6393

1

Immunoblotting Assays in Cellular Studies

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Monoclonal antibody against MAP1S (Cat# AG10006) was a gift from Precision Antibody, A&G Pharmaceutical, Inc., Columbia, Maryland. Primary antibodies against GFP (SC‐8334), β‐actin (SC‐47778), P27 (SC‐776), EEA1 (SC‐33585), Beclin 1 (SC‐11427), phosphorylated Bcl‐2 (p‐Bcl2, SC‐377576), phosphorylated p27 (p‐p27, SC‐130603), and ATG5 (SC‐33210) were purchased from Santa Cruz Biotechnology, Inc., Dallas, Texas, USA. Antibody against human LC3 (NB 100‐2331) was from Novus Biologicals. Antibodies against α‐SMA (ab‐5694), fibronectin (ab2413), LAMP2 (ab18528), and TGF‐β (ab66043) were from abcam, Cambridge, Massachusetts, USA. Antibodies against γ‐H2AX (9718 S), Bcl‐2 (#2870), PI3KCIII (#4263), H2AX (#2595), and ATG4B (#5299) were from Cell Signaling Technology, Danvers, Massachusetts, USA. Antibody against p62 (BML‐PW9860) was from Enzo Life Sciences International Plymouth Meeting, Pennsylvania, USA. Horseradish peroxidase‐conjugated secondary antibodies against mouse (#172‐1011) and rabbit (#172‐1019) were from Bio‐Rad, Hercules, California, USA. Rhodamine Red‐X goat anti‐mouse IgG (R6393) and FITC rabbit anti‐mouse IgG (A21202) were from Invitrogen, Carlsbad, California, USA. RFP‐LC3 was a gift from Dr. Mizushima (Mizushima et al., 2004). Bafilomycin A1, Sirius Red (Direct Red 80, 365548) and Hydroxyproline Assay Kit (MAK008‐1KT) were from Sigma, St. Louis, Missouri, USA.
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2

Monoclonal Antibody Characterization for Cell Biology

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Monoclonal antibody against MAP1S (Cat# AG10006) was a gift from Precision Antibody™, A&G Pharmaceutical, Inc.. Primary antibodies against β-actin (SC-47778) and GFP (SC-8334) were purchased from Santa Cruz Biotechnology, Inc.. Antibodies against fibronectin (ab2413), TGF-β (ab66043) and α-SMA (ab-5694) were from abcam. Horseradish peroxidase-conjugated secondary antibodies against mouse (#172-1011) and rabbit (#172-1019) were from Bio-Rad. Rhodamine Red-X goat anti-mouse IgG (R6393) and FITC rabbit anti-mouse IgG (A21202) were from Invitrogen. RFP-LC3 was a gift from Dr. Mizushima [34 (link)]. Antibody against caspase 1 (PRS3459), bafilomycin A1 and Sirius Red (Direct Red 80, 365548) were from Sigma.
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