HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed, #71-5500). β-actin was also detected as a loading control using a 1:10,000 dilution of mouse anti-β-actin (Sigma, A5441). A 1:3,000 dilution of goat anti-mouse (Dako, #P0447), anti-rabbit (Thermo Scientific, #P0448) or anti-chicken (Millipore, #12-341) horseradish peroxidase-conjugated antibody was used as a secondary probe. V5 tagged constructs were probed with anti-mouse V5 (Invitrogen #R960-25). Blots were developed using the ECL Plus detection reagent (GE Healthcare, #RPN2132). Antibodies to detect viral antigens, LGTV (NS3 and NS5) (previously described in Taylor et al., 2011 (link)), WNV-NS3 (R&D Systems, #AF2907) and dsRNA antibody J2 (English& Scientific Consulting, #10010200). Autophagy and cellular markers were detected using LC3B (Nanotools, #5F10), GABARAP (Cell Signaling, #E1J4E), Beclin-1 (Novus Biologicals, # 110-53818), ATG5 (Cell Signaling, #2630), p62 (BD Transduction Laboratories, #610833), cyclophilin A (Enzo, #BML-SA296-0100), cyclophilin B (Thermo Scientific, #PA1-027A), langerin (R&D Systems, #AF2088) and DC-SIGN (BD Biosciences, #551186).
Langerin
Manufactured by R&D Systems
Langerin is a cell surface receptor protein expressed on Langerhans cells in the skin and mucosa. It functions as a pattern recognition receptor and plays a role in the immune response.
Automatically generated - may contain errors
Lab products found in correlation
Cyclophilin b, by Thermo Fisher Scientific (2 mentions)
Mouse anti v5, by Thermo Fisher Scientific (2 mentions)
Ecl plus detection reagent, by GE Healthcare (2 mentions)
Beclin 1, by Novus Biologicals (2 mentions)
Dc sign, by BD (2 mentions)
Mouse anti β actin, by Merck Group (2 mentions)
β actin, by Merck Group (2 mentions)
Gabarap, by Cell Signaling Technology (2 mentions)
Cyclophilin a, by Enzo Life Sciences (2 mentions)
Wnv ns3, by R&D Systems (2 mentions)
Anti ha, by Zymo Research (2 mentions)
2 protocols using langerin
1
Western Blot and Immunofluorescence Antibody Detection
2
Western Blot and Immunofluorescence Antibody Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HA-tagged constructs for western blotting were detected using a 1:5000 dilution of anti-HA-peroxidase antibody (Roche clone 3F10, #12013819001). HA-tagged constructs for indirect immunofluorescence were detected using anti-HA (Zymed, #71-5500). β-actin was also detected as a loading control using a 1:10,000 dilution of mouse anti-β-actin (Sigma, A5441). A 1:3,000 dilution of goat anti-mouse (Dako, #P0447), anti-rabbit (Thermo Scientific, #P0448) or anti-chicken (Millipore, #12-341) horseradish peroxidase-conjugated antibody was used as a secondary probe. V5 tagged constructs were probed with anti-mouse V5 (Invitrogen #R960-25). Blots were developed using the ECL Plus detection reagent (GE Healthcare, #RPN2132). Antibodies to detect viral antigens, LGTV (NS3 and NS5) (previously described in Taylor et al., 2011 (link)), WNV-NS3 (R&D Systems, #AF2907) and dsRNA antibody J2 (English& Scientific Consulting, #10010200). Autophagy and cellular markers were detected using LC3B (Nanotools, #5F10), GABARAP (Cell Signaling, #E1J4E), Beclin-1 (Novus Biologicals, # 110-53818), ATG5 (Cell Signaling, #2630), p62 (BD Transduction Laboratories, #610833), cyclophilin A (Enzo, #BML-SA296-0100), cyclophilin B (Thermo Scientific, #PA1-027A), langerin (R&D Systems, #AF2088) and DC-SIGN (BD Biosciences, #551186).
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