The Sol muscle was isolated and immediately fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, post-fixed in buffered 1% osmium tetroxide containing 0.8% potassium ferricyanide, and enbloc stained in 1% aqueous uranyl acetate. They were then dehydrated with a graded series of acetonitrile and embedded in EMbed-812 resin. Thin sections (80 nm) were cut on a Leica EM UC6 ultramicrotome and stained with 2% uranyl acetate and lead citrate. Images were acquired using a Gatan US1000 2K CCD camera on FEI Tecnai G220 electron microscope equipped with a LaB6 source and operating at 100 kV or 200 kV.
Us1000 2k ccd camera
Manufactured by Thermo Fisher Scientific
Sourced in United States
The US1000 2K CCD camera is a high-resolution imaging device designed for scientific applications. It features a 2048 x 2048 pixel CCD sensor with a pixel size of 7.4 μm, providing a large field of view and high image quality. The camera offers a 16-bit digital output and a maximum frame rate of 10 Hz, making it suitable for a variety of imaging tasks that require detailed, high-quality data capture.
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Lab products found in correlation
2 protocols using us1000 2k ccd camera
1
Ultrastructural Analysis of Sol Muscle
2
Transmission Electron Microscopy of dHL-60 Cells
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Transmission electron microscopy was performed at the Purdue life science microscopy facility. dHL-60 cells were pelleted (14,000 g for 2 min) and fixed in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, post-fixed in buffered 1% osmium tetroxide containing 0.8% potassium ferricyanide, and en bloc stained in 1% aqueous uranyl acetate. They were then dehydrated with a graded series of acetonitrile and embedded in EMbed-812 resin. Thin sections (80 nm) were cut on a Leica EM UC6 ultramicrotome (Buffalo Grove, IL, USA) and stained with 2% uranyl acetate and lead citrate. Images were acquired using a Gatan US1000 2K CCD camera (Pleasanton, CA, USA) on a FEI Tecnai G2 20 electron microscope (Hillsboro, OR, USA) equipped with a LaB6 source and operating at 100 kV or 200 kV.
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