Cellular protein was extracted as described previously [23 (link)]. Antibodies against E2F1 (ab218527, Abcam, Cambridge, MA, USA), TXNIP (ab188865), ASK1 (sc-5294, Santa Cruz, CA, USA) and GAPDH (sc-25778) were used. GAPDH served as the internal control. ImageJ v1.50e was used to quantify the band intensity.
Sc 5294
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc-5294 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed to perform specific laboratory functions, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation on its intended use.
Automatically generated - may contain errors
3 protocols using sc 5294
1
Cellular Protein Extraction and Immunoblotting
2
Immunofluorescence Analysis of Cochlear Tissues
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The mice were euthanized with excessive CO 2 and their cochlear were removed. The cochlear were xed in 10% formaldehyde solution for 2 days after removing the auditory vesicles. 7% EDTA decalci ed solution at room temperature until cochlear osteomalacia. After dehydration in alcohol, the cochlear were cut into 5-μm paraff in sections that parallel to the axis of the cochlea. Primary antibodies were anti-Trx (Proteintech ® , 14999-1-AP, 1:200), anti-ASK1 (Santa Cruz, sc-5294, 1:200). Antigens were visualized after incubation with Alexa Fluor® 488 or 649-conjugated goat IgGs (Molecular Probes). Fluorescent images were captured using microscope (Leica DM6000B uorescence microscope, Germany).
3
Immunofluorescence Analysis of Cochlear Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mice were euthanized with excessive CO 2 and their cochlear were removed. The cochlear were xed in 10% formaldehyde solution for 2 days after removing the auditory vesicles. 7% EDTA decalci ed solution at room temperature until cochlear osteomalacia. After dehydration in alcohol, the cochlear were cut into 5-μm paraff in sections that parallel to the axis of the cochlea. Primary antibodies were anti-Trx (Proteintech ® , 14999-1-AP, 1:200), anti-ASK1 (Santa Cruz, sc-5294, 1:200). Antigens were visualized after incubation with Alexa Fluor® 488 or 649-conjugated goat IgGs (Molecular Probes). Fluorescent images were captured using microscope (Leica DM6000B uorescence microscope, Germany).
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