RPMI-1640 medium, Fetal bovine serum (FBS), and DMSO were purchased from Sigma (St. Louis, MO, USA). Caspase-Glo 3/7, Caspase-8, Caspase-9, and CellTiter 96 Aqueous kit were purchased from Promega (Madison, WI, USA). Propidium iodide (PI) and Alexa Fluor 488 Annexin V were purchased from Invitrogen (Waltham, MA, USA). Antibodies against PCNA, p21, p27, CDK2, Cdc25A, Cdc25C, CyclinA2, Cyclin E1, ATM, p-ATM (Ser1981), γ-H2AX (Ser139), PARP, Fas, DR5, FADD, Bax, Bcl-2, Bcl-xL, Apaf-1, Cytc, p53, p-p53 (Ser15), AKT, p-AKT (Ser473), and MDM2 were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against Chk2, p-Chk2 (Thr 68), Bad, and GAPDH were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
Caspase 9
Manufactured by Promega
Sourced in United States
Caspase-9 is a key enzyme involved in the intrinsic apoptotic pathway. It plays a central role in the initiation of programmed cell death. Caspase-9 is responsible for the activation of downstream effector caspases, leading to the execution of the apoptotic process.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5 alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Cyt c, by Cell Signaling Technology (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
γh2ax, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
Caspase 8, by Promega (1 mentions)
Apaf 1, by Cell Signaling Technology (1 mentions)
P p53 ser15, by Cell Signaling Technology (1 mentions)
Celltiter 96 aqueous kit, by Promega (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Caspase glo 3 7, by Promega (1 mentions)
2 protocols using caspase 9
1
Apoptosis-Inducing Compound Screening Protocol
2
Caspase-8 and Caspase-9 Activities in Multiple Myeloma
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Caspase-8 and caspase-9 activities were assessed in all 6 Multiple Myeloma cell lines.
Cells were treated with the pre-determined dose of TRAIL in the presence or absence of GSK343 (0, 5, 10 and 15µM) in triplicate wells of in white, Fisher Scientific 96 well plates. Cells were lysed as the Caspase-Glo® reagent was added the assay was performed following the manufacturer's instructions with 24-hour incubation in Caspase-Glo reagent. 50µl of either caspase-8 reagent or caspase-9 (Promega) reagent was then added with MG132 proteosome inhibitor. The samples were then incubated at room temperature for 90 minutes before the Wallac Victor 2 1420 luminometer was used to measure the luminescence.
Cells were treated with the pre-determined dose of TRAIL in the presence or absence of GSK343 (0, 5, 10 and 15µM) in triplicate wells of in white, Fisher Scientific 96 well plates. Cells were lysed as the Caspase-Glo® reagent was added the assay was performed following the manufacturer's instructions with 24-hour incubation in Caspase-Glo reagent. 50µl of either caspase-8 reagent or caspase-9 (Promega) reagent was then added with MG132 proteosome inhibitor. The samples were then incubated at room temperature for 90 minutes before the Wallac Victor 2 1420 luminometer was used to measure the luminescence.
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