Methiothepin was purchased from Sigma-Aldrich (St.Louis, MO, USA). Z-VAD FMK was purchased from Sigma-Aldrich. Antibodies against MCL-1, BCL-2, BCL-XL, PARP, GRP78/Bip, phosphorylated PERK (p-PERK), ATF4, and CHOP were obtained from Cell Signaling Technology (Danvers, MA, USA). Paclitaxel was purchased from Sigma-Aldrich.
Methiothepin
Manufactured by Merck Group
Sourced in United States
Methiothepin is a laboratory reagent used in biological and pharmacological research. It is a serotonin receptor antagonist that can be utilized for the study of serotonergic systems. The core function of Methiothepin is to serve as a tool for investigating the role of serotonin receptors in various physiological and pathological processes.
Automatically generated - may contain errors
Lab products found in correlation
Paclitaxel, by Merck Group (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
P perk, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Mcl 1, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Clozapine, by Merck Group (1 mentions)
Sb 269970, by Abcam (1 mentions)
Ketanserin, by MedChemExpress (1 mentions)
Norepinephrine ne hydrochloride, by Merck Group (1 mentions)
L arginine, by Merck Group (1 mentions)
Glibenclamide, by Merck Group (1 mentions)
Paracetamol, by Merck Group (1 mentions)
β caryophyllene oxide, by Merck Group (1 mentions)
Oxadiazolo 4 3 a quinoxalin 1 one odq, by Merck Group (1 mentions)
Ng nitro l arginine methyl ester hydrochloride l name, by Merck Group (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Phenol red, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium f12, by Merck Group (1 mentions)
Way100635, by Selleck Chemicals (1 mentions)
6 protocols using methiothepin
1
Apoptosis Regulation by Methiothepin
2
Pharmacological Validation of 5-HT7 Receptor
3
Pharmacological Modulation of Nociception
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Paracetamol, β-caryophyllene oxide, methiothepin, glibenclamide, [1 ,2 (link),4 (link)] oxadiazolo [4,3-a]quinoxalin-1-one (ODQ), N(G)-nitro-l -arginine methyl ester hydrochloride (l -NAME), and l -arginine were obtained from Sigma-Aldrich (St. Louis, MO, USA), and naloxone was acquired from Pisa Laboratories (Mexico City, Mexico). All drugs were suspended in carboxymethylcellulose (0.1%) and prepared minutes before being given to the animals. β-caryophyllene oxide and Paracetamol were administered orally (0.1 mL/10 g of body weight). Naloxone, methiothepin, l -NAME, ODQ, l -arginine, and glibenclamide were injected intraperitoneally (0.1 mL/10 g of body weight). Formalin (2%) was prepared by diluting aqueous formaldehyde to 37% from J.T. Baker (Matsonford Rd. Radnor Township, PA, USA).
4
GPCR Signaling Pathway Assays
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(+/−)-1-(2,5-Dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI), 8-methoxy-2-tetralone (8-OH-DPAT), methiothepin, katanserin and Dulbecco's modified Eagle's/F12 medium (50:50, v/v) without phenol red were purchased from Sigma-Aldrich (St. Louis, MO). 5-HT was obtained from Santa Cruz Biotechnology (Santa Cruz, Inc., CA). SB269970 and WAY100635 were purchased from Selleck (Houston, USA). 5-HT1A and Gα16 plasmids were obtained from Bioworld (Minnesota, USA). pUNIV-5-HT2A was provided by Professor Jeanne Mialet-Perez (The cDNA Resource Center of Missouri-Rolla University, France). pCDNA3-5-HT7A was a gift from professor Kathleen Van Craenenbroeck (Ghent University, Belgium). The pCRE-Luc vector was purchased from Clontech (BD Biosciences Clontech, CA). The pGL3-NFAT-luc vector was kindly provided by Professor Karine Lefort (University of Lausanne, Switzerland), and the SRE-luc vector was kindly provided by professor Jae Young Seong (University College of Medicine, Korea). Dual Luciferase Assay kits were purchased from Promega (Madison, WI). Carbon absorption foetal bovine serum and Lipofectamine 2000 were purchased from Invitrogen (Carlsbad, CA). The CHO-K1 cells and 293T cells were obtained from the Institute of Biochemistry and Cell Biochemistry and Cell Biology (Shanghai, China). White opaque 96-well microplates were obtained from Corning (Acton, MA, USA).
5
Synthesis and Evaluation of JJGW08 Compound
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The studied compound 2-{5-[4-(2-methoxyphenyl)piperazin-1-yl]pentoxy}benzamide hydrochloride (JJGW08) was synthesized in the Department of Organic Chemistry and Technology, Faculty of Chemical and Engineering and Technology, Cracow University of Technology. The synthesis and biological properties of the compound were described earlier [52 (link)].
JJGW08 was dissolved in saline (0.9 % NaCl, Polpharma, Starogard Gdańsk, Poland) and administered intraperitoneally (ip) 30 min before each behavioral test. The chemicals used in radioligand studies, i.e., methiothepin (Sigma-Aldrich, Darmstadt, Germany), were dissolved in saline. MK-801 (Sigma-Aldrich, Darmstadt, Germany) was dissolved in saline and administered ip 15 min before experiments. The control groups received 0.9% NaCl solution ip 30 min prior to testing. The studied compound was tested in in vivo experiments at the dose range of 0.625–2.5 mg/kg, which was selected based on our previous experiments [53 (link)]. If the effect of the lowest dose, i.e., 0.625 mg/kg, was still statistically significant, we reduced the dose by half until the observed activity disappeared.
JJGW08 was dissolved in saline (0.9 % NaCl, Polpharma, Starogard Gdańsk, Poland) and administered intraperitoneally (ip) 30 min before each behavioral test. The chemicals used in radioligand studies, i.e., methiothepin (Sigma-Aldrich, Darmstadt, Germany), were dissolved in saline. MK-801 (Sigma-Aldrich, Darmstadt, Germany) was dissolved in saline and administered ip 15 min before experiments. The control groups received 0.9% NaCl solution ip 30 min prior to testing. The studied compound was tested in in vivo experiments at the dose range of 0.625–2.5 mg/kg, which was selected based on our previous experiments [53 (link)]. If the effect of the lowest dose, i.e., 0.625 mg/kg, was still statistically significant, we reduced the dose by half until the observed activity disappeared.
6
Cardamonin-PCPA Interaction Protocol
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Cardamonin or 2,4-dihydroxy-6-methoxyphenyl)-3-phenyl-2-propen-1-one with ≥98% purity and PCPA, ρ-chlorophenylalanine (serotonin synthesis inhibitor) were obtained from Cayman Chemical (Ann Arbor, MI, USA) and HiMedia (Mumbai, India), respectively. Tween 20, 5% DMSO, tribromoethanol, normal saline (0.9% NaCl), iodine solution, and serotonin antagonist subtypes: methiothepin, ketanserin, ondansetron, and amitriptyline were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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