Gradient tgx precast gels

Manufactured by Bio-Rad

4–15% gradient TGX precast gels are protein electrophoresis gels designed for the separation of proteins. They feature a 4–15% polyacrylamide gradient for the separation of a wide range of protein molecular weights.

Automatically generated - may contain errors

Lab products found in correlation

Ecl lightening plus, by PerkinElmer (1 mentions) Nitrocellulose, by Cytiva (1 mentions)

Close spelling variants of this product

TGX Stain-Free Precast gradient gels Gradient Mini-PROTEAN TGX precast protein gels Mini-PROTEAN® TGX™ precast 4–15% gradient gels Mini protean TGX precast 4-20% gradient gels TGX precast gels 4–20% gradient TGX gels 4–20% TGX gradient gel Precast gradient gels TGX gels Precast gels

2 protocols using gradient tgx precast gels

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue was lysed using RIPA buffer (50mM Tris, pH 7.4, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) supplemented with protease inhibitors (Calbiochem, Billerica, MA). Protein samples were separated on 4–15% gradient TGX precast gels (Bio-Rad, Hercules, CA), transferred to nitrocellulose membrane (Whattman, Pittsburgh, PA). Membranes were blocked and antibodies were diluted in 5% milk in TBST (50mM Tris, pH 7.6, 150mM NaCl, 0.1% Tween 20) and analyzed using ECL Lightening Plus (Perkin–Elmer, Waltham, MA).

Sigg M.A., Menchen T., Lee C., Johnson J., Jungnickel M.K., Choksi S.P., Garcia G 3.r.d., Busengdal H., Dougherty G., Pennekamp P., Werner C., Rentzsch F., Florman H.M., Krogan N., Wallingford J.B., Omran H, & Reiter J.F. (2017). Evolutionary proteomics uncovers ancient associations of cilia with signaling pathways. Developmental cell, 43(6), 744-762.e11.

+ Open protocol

+ Expand

Immunoblot Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunoblots were performed as described previously (Raleigh et al., 2018 (link)). In brief, samples were boiled in 2x Laemmli reducing buffer for 5 min, separated on 4%–15% gradient TGX precast gels (Bio-Rad), transferred onto nitrocellulose (Whatman, Pittsburgh, PA) and subjected to immunoblot.

Raleigh D.R., Sever N., Choksi P.K., Sigg M.A., Hines K.M., Thompson B.M., Elnatan D., Jaishankar P., Bisignano P., Garcia-Gonzalo F.R., Krup A.L., Eberl M., Wong S.Y., Renslo A.R., Grabe M., McDonald J.G., Xu L., Beachy P.A, & Reiter J.F. (2018). Cilia-associated oxysterols activate Smoothened. Molecular cell, 72(2), 316-327.e5.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!