Jurkat

Manufactured by Shanghai Cell Bank

Sourced in China

Jurkat is a T-cell line derived from human T-cell leukemia. It is a widely used cell line in immunological and cancer research studies.

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Lab products found in correlation

Kasumi 1, by Shanghai Cell Bank (1 mentions) Su dhl 4, by Shanghai Cell Bank (1 mentions) Thp 1, by Shanghai Cell Bank (1 mentions) Hl 60, by Shanghai Cell Bank (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

2 protocols using jurkat

Cultivation of Hematological Cancer Cell Lines

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293T/17, Namalwa (human Burkitt lymphoma cell), Karpas-299 (human anaplastic large cell lymphoma cells), Daudi (human Burkitt lymphoma cells), HL-60 (human myeloid leukemia cells), Su-DHL-4 (human diffuse large B-cell lymphoma cells), Kasumi-1 (human acute myeloid leukemia cells), HEL (human erythroleukemia leukemia cells), K562 (human chronic myeloid leukemia cells), THP1 (human monocyte leukemia cells) and Jurkat (human T lymphocytic leukemia cells) cell lines were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). The lentivirus packaging cell line 293T/17 was maintained in Dulbecco's modified Eagle's medium (DMEM; Hyclone; GE Healthcare Life Sciences, Logan, UT, USA). The remaining hematological tumor cells were cultured in Iscove's modified Dulbecco's medium (IMDM) or RPMI-1640 medium (Hyclone; GE Healthcare Life Sciences). Cell cultures were supplemented with 0.1 U/ml streptomycin, 0.1 U/µl penicillin and 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The cells were cultured at 37°C in a humidified atmosphere of 5% CO₂.

Yang M., Tian X., Fan Z., Yu W., Li Z., Zhou J., Zhang W, & Liang A. (2019). Targeting RAD51 enhances chemosensitivity of adult T-cell leukemia-lymphoma cells by reducing DNA double-strand break repair. Oncology Reports, 42(6), 2426-2434.

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Cell Line Authentication and Mycoplasma Testing

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HeLa, K562, Jurkat, and NCI-H929 cells with STR profiling were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China) or ATCC and stored in-house. Cell lines were cultured in DMEM or RPMI-1640 medium (HyClone) supplemented with 10% FBS (HyClone) and 2 mmol/L l-glutamine (Gibco), and checked for the absence of Mycoplasma by PCR.

Cheng J., Zhang G., Deng T., Liu Z., Zhang M., Zhang P., Adeshakin F.O., Niu X., Yan D., Wan X, & Yu G. (2023). CD317 maintains proteostasis and cell survival in response to proteasome inhibitors by targeting calnexin for RACK1-mediated autophagic degradation. Cell Death & Disease, 14(5), 333.

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