General chemicals were from VWR (West Chester, PA, USA) and Sigma Aldrich (St. Louis, MO, USA). Phorbol myristate acetate (PMA) and ionomycin were from Calbiochem (Gibbstown, NJ, USA). IgE anti-di-nitro phenol (anti-DNP) is from Sigma and KLH-DNP was from Calbiochem. Bee venom was from HollisterStier (Spokane, WA, USA). To mitigate batch-to-batch variation in venom, three independent batches were selected on the basis of similar potency for induction of histamine release in control experiments, mixed, aliquoted and used for the duration of the studies presented here. Mastoparan and mellitin were from Sigma Aldrich. Arachidonic acid was from Enzo (Farmingdale, NY, USA). 2-Aminoethoxydiphenyl borate (2-APB) was from Calbiochem (LaJolla, CA, USA). Capsaicin and capsazepine were from Sigma Aldrich.
Arachidonic acid
Manufactured by Enzo Life Sciences
Sourced in United States, Germany
Arachidonic acid is a polyunsaturated fatty acid that is a key component of cell membranes. It plays a crucial role in various physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Mellitin, by Merck Group (1 mentions)
Mastoparan, by Merck Group (1 mentions)
Capsaicin, by Merck Group (1 mentions)
Capsazepine, by Merck Group (1 mentions)
Sb203580, by Enzo Life Sciences (1 mentions)
Sp600125, by Enzo Life Sciences (1 mentions)
Win55 212 2, by R&D Systems (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Pd98059, by R&D Systems (1 mentions)
Fetal calf serum (fcs), by PAN Biotech (1 mentions)
2 protocols using arachidonic acid
1
Induction of Histamine Release by Bee Venom
2
Cannabinoid Signaling Pathway Analysis
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WIN 55,212-2, JWH-133, cannabidiol, MA and PD98059 were purchased from R&D Systems, Inc. (Wiesbaden-Nordenstadt, Germany). WIN 55,212-3 was from LGC Standards (Wesel, Germany). 2-AG was obtained from Cayman Chemical (Ann Arbor; Michigan, USA). AM-251, AM-630, AEA, arachidonic acid, SB203580 and SP600125 were bought from Enzo Life Sciences (Lörrach, Germany). Capsazepine, indometacin, myriocin from Mycelia sterilia (ISP-1), C2-ceramide (N-acetyl-D-sphingosine), dihydro-C2-ceramide (D-erythro-N-acetylsphinganine) and nSMase from Bacillus cereus were obtained from Sigma-Aldrich (Taufkirchen, Germany). IL-1β, penicillin-streptomycin and trypsin/EDTA were from Invitrogen Life Technologies (Darmstadt, Germany). THC and nSMase spiroepoxide inhibitor were from Lipomed (Weil am Rhein, Germany) and Santa Cruz Biotechnology (Heidelberg, Germany), respectively. Fumonisine B1 from Fusarium monoliforme was from Merck Millipore (Darmstadt, Germany). Fetal calf serum (FCS) was obtained from PAN Biotech GmbH (Aidenbach, Germany).
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