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Anti spike s2

Manufactured by Thermo Fisher Scientific
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The Anti-Spike S2 is a laboratory instrument designed to detect and quantify the presence of the SARS-CoV-2 spike protein S2 subunit in samples. It utilizes advanced detection techniques to provide accurate and reliable data on the target analyte.

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Lab products found in correlation

Chemidoc mp imaging system, by Bio-Rad (3 mentions) Sds 4 to 12 bis tris protein gels, by Thermo Fisher Scientific (3 mentions)

3 protocols using anti spike s2

1

SARS-CoV-2 Spike Protein Characterization

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Forty-eight hours after transfection of cells with plasmid preparations, the culture supernatant was harvested and passed through a 0.45-μm-pore-size filter to remove cellular debris. The filtrate was centrifuged at 15,000 rpm for 120 min to pellet virions. The pelleted virions were lysed in Laemmli reducing buffer (1 M Tris-HCl [pH 6.8], SDS, 100% glycerol, β-mercaptoethanol, and bromophenol blue). Pelleted virions were subjected to electrophoresis on SDS–4 to 12% bis-Tris protein gels (Thermo Fisher Scientific) under reducing conditions. This was followed by electroblotting onto polyvinylidene difluoride (PVDF) membranes. The SARS-CoV-2 Spike proteins were visualized by a ChemiDoc> MP imaging system (Biorad) using anti-Spike S2 (Invitrogen at 1:1000 dilution) and anti-p24 Gag antibodies (NIH AIDS Reagents 1:1000 dilution).
Kemp S.A., Collier D.A., Datir R.P., Ferreira I.A., Gayed S., Jahun A., Hosmillo M., Rees-Spear C., Mlcochova P., Lumb I.U., Roberts D.J., Chandra A., Temperton N., Sharrocks K., Blane E., Modis Y., Leigh K., Briggs J., van Gils M., Smith K.G., Bradley J.R., Smith C., Doffinger R., Ceron-Gutierrez L., Barcenas-Morales G., Pollock D.D., Goldstein R.A., Smielewska A., Skittrall J.P., Gouliouris T., Goodfellow I.G., Gkrania-Klotsas E., Illingworth C.J., McCoy L.E, & Gupta R.K. (2021). SARS-CoV-2 evolution during treatment of chronic infection. Nature, 592(7853), 277-282.
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2

SARS-CoV-2 Spike Protein Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Forty-eight hours after transfection of cells with plasmid preparations, the culture supernatant was harvested and passed through a 0.45-μm-pore-size filter to remove cellular debris. The filtrate was centrifuged at 15,000 rpm for 120 min to pellet virions. The pelleted virions were lysed in Laemmli reducing buffer (1 M Tris-HCl [pH 6.8], SDS, 100% glycerol, β-mercaptoethanol, and bromophenol blue). Pelleted virions were subjected to electrophoresis on SDS–4 to 12% bis-Tris protein gels (Thermo Fisher Scientific) under reducing conditions. This was followed by electroblotting onto polyvinylidene difluoride (PVDF) membranes. The SARS-CoV-2 Spike proteins were visualized by a ChemiDoc> MP imaging system (Biorad) using anti-Spike S2 (Invitrogen at 1:1000 dilution) and anti-p24 Gag antibodies (NIH AIDS Reagents 1:1000 dilution).
Kemp S.A., Collier D.A., Datir R.P., Ferreira I.A., Gayed S., Jahun A., Hosmillo M., Rees-Spear C., Mlcochova P., Lumb I.U., Roberts D.J., Chandra A., Temperton N., Sharrocks K., Blane E., Modis Y., Leigh K., Briggs J., van Gils M., Smith K.G., Bradley J.R., Smith C., Doffinger R., Ceron-Gutierrez L., Barcenas-Morales G., Pollock D.D., Goldstein R.A., Smielewska A., Skittrall J.P., Gouliouris T., Goodfellow I.G., Gkrania-Klotsas E., Illingworth C.J., McCoy L.E, & Gupta R.K. (2021). SARS-CoV-2 evolution during treatment of chronic infection. Nature, 592(7853), 277-282.
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3

Immunoblotting Analysis of SARS-CoV-2 Spike Proteins

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Forty-eight hours after transfection of cells with plasmid preparations, the culture supernatant was harvested and passed through a 0.45-μm-pore-size filter to remove cellular debris. The filtrate was centrifuged at 15,000 rpm for 120 min to pellet virions. The pelleted virions were lysed in Laemmli reducing buffer (1 M Tris-HCl [pH 6.8], SDS, 100% glycerol, β-mercaptoethanol, and bromophenol blue). Pelleted virions were subjected to electrophoresis on SDS–4 to 12% bis-Tris protein gels (Thermo Fisher Scientific) under reducing conditions. This was followed by electroblotting onto polyvinylidene difluoride (PVDF) membranes. The SARSCOV-2 Spike proteins were visualized by a ChemiDoc® MP imaging system (Biorad) using anti-Spike S2 (Invitrogen) and anti-p24 Gag antibodies.
Kemp S., Collier D., Datir R., Ferreira I., Gayed S., Jahun A., Hosmillo M., Rees-Spear C., Mlcochova P., Lumb I.U., Roberts D.J., Chandra A., Temperton N., Sharrocks K., Blane E., Briggs J., van G.M., Smith K., Bradley J., Smith C., Doffinger R., Ceron-Gutierrez L., Barcenas-Morales G., Pollock D., Goldstein R., Smielewska A., Skittrall J., Gouliouris T., Goodfellow I., Gkrania-Klotsas E., Illingworth C., McCoy L, & Gupta R. (2020). Neutralising antibodies in Spike mediated SARS-CoV-2 adaptation. medRxiv.
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