Bcl 2 human sandwich elisa kit

Ten gm placental specimens were washed three times with normal saline and twice with a phosphate buffer solution (PBS). After the aspiration of the PBS, a lysis solution was added (2 mL) then specimens were homogenized, and gently shaken for 1 h. The homogenates were then centrifuged for 10 min at 1500 rpm and the lysates were kept preserved in aliquots at – 80 °C still being used.
The total placental protein concentration was measured after the procedure of Lowry et al.^{14 (link)} using the Folin phenol reagent. Placental Bcl-2 and caspase-3 levels were measured by an Invitrogen™ Bcl-2 human sandwich ELISA Kit, and caspase-3 Human Instant ELISA Kit (Thermo Fisher Scientific Inc, A-1030 Vienna, Austria cat# BMS244-3, and BMS2012INST, respectively). The levels were expressed as ng/mg protein in placental tissue extract.

Ten gm placental specimens were washed three times with normal saline and twice with a phosphate buffer solution (PBS). After the aspiration of the PBS, a lysis solution was added (2 mL) then specimens were homogenized, and gently shaken for 1 h. The homogenates were then centrifuged for 10 min at 1500 rpm and the lysates were kept preserved in aliquots at – 80 °C still being used.
The total placental protein concentration was measured after the procedure of Lowry et al.^{14 (link)} using the Folin phenol reagent. Placental Bcl-2 and caspase-3 levels were measured by an Invitrogen™ Bcl-2 human sandwich ELISA Kit, and caspase-3 Human Instant ELISA Kit (Thermo Fisher Scientific Inc, A-1030 Vienna, Austria cat# BMS244-3, and BMS2012INST, respectively). The levels were expressed as ng/mg protein in placental tissue extract.