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Bac t alert 3d blood culture instrument

Manufactured by bioMérieux
Sourced in United States, Denmark

The BAC-T/ALERT®3D Blood Culture Instrument is a laboratory equipment used for the automated detection and monitoring of microbial growth in blood culture samples. The instrument employs a colorimetric detection system to identify the presence of microorganisms in the samples.

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2 protocols using bac t alert 3d blood culture instrument

1

Blood Culture Procedures for Microbial Detection

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In the laboratory, BAC-T/ALERT®3D Blood Culture Instrument and BACT/Alert FA (BioMérieux, Durham, NC, USA) were used for inoculation and anaerobic cultures were obtained using blood agar, MacConkey agar, chocolate agar, and Schindler’s agar. Sabouraud agar was used for fungal cultures.
Potassium, albumin, B12 and microbial culture were measured on the day of admission.
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2

Defining Ventilator-Associated Pneumonia and Bloodstream Infections

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VAP was defined as new or changing chest X-ray infiltrate/s occurring more than 48 h after initiation of invasive mechanical ventilation, plus both of the following: (i) new onset of fever (body temperature ≥ 38 °C)/hypothermia (body temperature ≤ 35 °C) and/or leukocytosis (total peripheral white blood cell count ≥ 10,000 cells/µL)/leukopenia (total WBC count ≤ 4500 cells/µL)/ > 15% immature neutrophils; (ii) new onset of suctioned respiratory secretions and/or need for acute ventilator support system changes to enhance oxygenation [13 (link)].
Catheter-related bloodstream infection (CRBSI) was defined as the presence of bacteremia originating from an intravenous catheter. Microbiological samples were performed using BacT/ALERT SA (aerobic) and BacT/ALERT SN (anaerobic) bottles incubated in the BacT/Alert 3D blood culture instrument (bioMérieux, Ballerup, Denmark) [14 (link)].
Fungal infection was diagnosed as previously described [15 (link)].
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