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2 protocols using lamp1 l1418

1

Antibody Selection and Validation

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The sources of purchased antibodies were as follows: FLT3 (S-18), FLT3 (SF1.340), ERK (K-23), and STAT5 (C-17) from Santa Cruz Biotechnology (Dallas, TX); FLT3 (8F2), FLT3[pY842] (10A8), FLT3[pY591] (54H1), AKT (40D4), AKT[pT308] (C31E5E), STAT5 (D2O6Y), STAT5[pY694] (D47E7), ERK1/2 (137F5), and ERK[pT202/pY204] (E10) from Cell Signaling Technology (Danvers, MA); TfR (ab84036), TGN46 (ab76282), and GM130 (EP892Y) from Abcam (Cambridge, UK); STAT5 (89) from BD Transduction Laboratories (Franklin Lakes, NJ); Calnexin (ADI-SPA-860) from Enzo (Farmingdale, NY); LAMP1 (L1418) from Sigma (St. Louis, MO) and FLT3 (MAB812) from R&D Systems (Minneapolis, MN). Horseradish peroxidase-labeled (HRP-labeled) donkey anti-mouse IgG and anti-rabbit IgG secondary antibodies were purchased from The Jackson Laboratory (Bar Harbor, MA). Alexa Fluor-conjugated (AF-conjugated) donkey secondary antibodies were obtained from Thermo Fisher Scientific (Rockford, IL). The list of antibodies with sources and conditions of immunoblotting and immunofluorescence is shown in Suppl. Table 1.
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2

Antibody Sources for Cell Signaling

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The sources of purchased antibodies were as follows: KIT (M− 14), cathepsin D (H− 75), STAT5 (C-17), ERK2 (K-23), ARF1 (ARFS 3F1), GBF1 (25), PTP1B (D-4), SHP-1 (D-11), and SHP-2 (B-1) from Santa Cruz Biotechnology (Dallas, TX); KIT [pY703] (D12E12), KIT (D13A2), LAMP1 (lysosome-associated membrane protein 1, D4O1S), AKT (40D4), AKT [pT308] (C31E5E), STAT5 (D2O6Y), STAT5[pY694] (D47E7), ERK1/2 (137F5) and ERK [pT202/pY204] (E10) from Cell Signaling Technology (Danvers, MA); PDI (RL90), TFR (transferrin receptor, ab84036), giantin (ab24586), and GM130 (EP892Y) from Abcam (Cambridge, UK); TFR (H68.4) from Thermo Fisher Scientific (Rockford, IL); calnexin (ADI-SPA-860) from Enzo (Farmingdale, NY); GM130 (clone 35) from BD Transduction Laboratories (Franklin Lakes, NJ); LAMP1 (L1418) from Sigma (St. Louis, MO) and KIT (104D2) from Biolegend (San Diego, CA). HRP-labeled secondary antibodies were purchased from the Jackson Laboratory (Bar Harbor, MA). Alexa Fluor-conjugated secondary antibodies were obtained from Molecular Probes (Eugene, OR).
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