For flow cytometry analyses of mouse PB, BM, Spleen, and Liver, hematopoietic cells were labeled with the following antibodies from Biolegend (San Diego CA) unless specifically indicated: Anti-CD3e-PE/Cyanine5 (mouse, #100310, 1:200 dilution), Anti-Ly-6G/Ly-6C (Gr-1)-PE/Cyanine5 (mouse, #108410, 1:200 dilution), Anti-CD11b-PE/Cyanine5 (mouse, #101210, 1:200 dilution), Anti-CD45R-PE/Cyanine5 (mouse, #103210, 1:200 dilution), Anti-Ter-119-PE/Cyanine5 (mouse, #116210, 1:200 dilution), Anti-CD117 (c-Kit)-APC (mouse, #105812, 1:200 dilution), Anti-Sca-1-PE-cy7 (mouse, #108114, 1:200 dilution), Anti-CD150-PE (mouse, #115904, 1:200 dilution), Anti-CD48-APC/Cyanine7 (mouse, #103432, 1:200 dilution), Anti-Ki67-FITC (mouse, #652410, 1:200 dilution), Hoechst34580 (#565877, BD Pharmingen), Anti-CD16/32-PE (mouse, #101308, 1:200 dilution), Anti-CD34-FITC (mouse, #11-0341-82, 1:200 dilution eBioscience), Anti-CD127-APC/Cyanine7 (mouse, #135040, 1:200 dilution), Anti-CD135-Brilliant Violet421 (mouse, #135314, 1:200 dilution), Annexin V (#640941, 1:20 dilution), PI (#421301, 1:50). Intracellular staining was performed using the Foxp3/Transcription factor staining set (eBioscience, Grand Island NY) and Fixation/Methanol protocol (eBioscience, Grand Island NY) provided by the manufacturers.
Anti cd16 32 pe
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-CD16/32-PE is a fluorescently-labeled monoclonal antibody that binds to the CD16 and CD32 cell surface antigens. It is commonly used in flow cytometry applications to identify and characterize immune cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5, by Thermo Fisher Scientific (1 mentions)
Anti cd34 fitc, by Thermo Fisher Scientific (1 mentions)
Anti ki67 fitc, by BD (1 mentions)
Hoechst 34580, by BD (1 mentions)
Anti sca1 pe cy7, by BD (1 mentions)
Foxp3 transcription factor staining set, by Thermo Fisher Scientific (1 mentions)
Fixation methanol protocol, by Thermo Fisher Scientific (1 mentions)
Antibody conjugation kit, by Thermo Fisher Scientific (1 mentions)
Anti cd64 pe, by Thermo Fisher Scientific (1 mentions)
Facscalibur, by BD (1 mentions)
Streptavidin apc, by BD (1 mentions)
2 protocols using anti cd16 32 pe
1
Comprehensive Flow Cytometry Analysis of Mouse Hematopoietic Cells
2
Blocking Fc Receptor Binding on Cells
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Binding to FcRs (CD16/32) was blocked by incubating the cells on ice for 30 min with 2.4G2 hybridoma supernatant, except in experiments where the FcRs were stained when the blocking was performed by incubating the cells in 5% BSA in FACS wash solution (0.5% BSA, 2 mM NaN3, 2 mM EDTA in PBS). After blocking, the antibodies were added for 1 h: anti-Sn-biotin (3D6 and SER-4 mAbs produced in-house); anti-TIM-4-PE, anti-CD16/32-PE, anti-CD64-PE (all eBioscience); anti-FcγRIIB Ly17.2 (kind gift from Prof. J. Ravetch, The Rockefeller University, NY, USA) that was directly conjugated to Alexa 488 fluorescent dye using an antibody conjugation kit (Invitrogen). Corresponding isotypes were used at the same concentration in each staining. After incubating the cells with antibodies, the cells were washed in FACS buffer and, if necessary, incubated with streptavidin-APC (BD Bioscience, San Jose, CA, USA) for a further 30 min on ice and washed again. Cells were fixed with 1% formaldehyde in PBS and data were acquired with FACSCalibur (BD Bioscience). Analysis was performed using FlowJo 7.6.4 (Tree Star).
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