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S1200ex

Manufactured by Stratedigm
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The S1200Ex is a flow cytometer instrument designed for cell analysis and sorting. It provides high-performance capabilities for a wide range of applications, including cell-based assays, immunophenotyping, and stem cell research.

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Lab products found in correlation

Macsmix tube rotator, by Miltenyi Biotec (1 mentions) Gentlemacs dissociator, by Miltenyi Biotec (1 mentions) Ic fixation buffer, by Thermo Fisher Scientific (1 mentions) Cellcapture software, by Stratedigm (1 mentions) Anti cd16 32 trustain fcx, by BioLegend (1 mentions) Brilliant stain buffer plus, by BD (1 mentions)

4 protocols using s1200ex

1

Isolation of NK Cells from Rat Colon Tumors

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At the termination of the experiment, rat colon tumors were dissociated for isolation of NK cells using tumor dissociation kits, a gentleMACS Dissociator and a MACSmixTM Tube Rotator according to manufacturer’s instructions (Miltenyi Biotech, CA). The NK cells and NK cells expressing perforin/IFN-γ were determined in dissociated tumor samples using antibodies to NK1.1, NKG2D, perforin, and IFN-γ. The single cell suspensions were stained as reported previously by us48 (link) and was run on a Stratedigm S1200Ex flow cytometer and data was acquired using the CellCapTure acquisition and analysis software.
Janakiram N.B., Mohammed A., Bryant T., Zhang Y., Brewer M., Duff A., Biddick L., Singh A., Lightfoot S., Steele V.E, & Rao C.V. (2016). Potentiating NK cell activity by combination of Rosuvastatin and Difluoromethylornithine for effective chemopreventive efficacy against Colon Cancer. Scientific Reports, 6, 37046.
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2

ILC Apoptosis Profiling with Annexin V and 7-AAD

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Apoptosis was monitored by Annexin V and 7-AAD staining following manufacturers’ protocols (BD Biosciences or BioLegend). Cells were treated or not with dexamethasone and IL-23 and after 6 hrs, cells were centrifuged and the supernatant was removed and stored. Cell pellets were washed with PBS. Cells were surface stained to identify ILCs. Cells were then stained with Annexin V and 7-AAD. Stained cells were immediately analyzed by flow cytometry on a Stratedigm S1200Ex flow cytometer (Stratedigm, San Jose, CA) and data were analyzed using FlowJo v.9.6 (Tree Star; Ashland, OR).
Seshadri S., Pope R.L, & Zenewicz L.A. (2018). Glucocorticoids inhibit group 3 innate lymphocyte IL-22 production. Journal of immunology (Baltimore, Md. : 1950), 201(4), 1267-1274.
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3

Surface Protein Expression Analysis

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Detached cells were washed by ice-cold PBS twice and incubated with 15% goat serum on ice for 1 hour. The cells were neither fixed nor permeabilized. Then, the cells were stained with primary Abs against the extracellular domain of the target protein for 1 hour and secondary Abs for 1 hour sequentially on ice, followed by washes with ice-cold PBS and centrifugation at 4°C. When lysosome was investigated, detached cells were incubated with 1μM Lysotracker at 37°C for 30 minutes. Expression levels were detected by using the Stratedigm S1200Ex or Stratedigm S1400Exi flow cytometer platform (Stratedigm Inc.) and analyzed by FlowJo software (BD Science).
Wang J., Wren J.D., Ding Y., Chen J., Mittal N., Xu C., Li X., Zeng C., Wang M., Shi J., Zhang Y.H., Han S.J, & Zhang X.A. (2022). EWI2 Promotes Endolysosome-mediated Turnover of Growth Factor Receptors and Integrins to Suppress Lung Cancer. Cancer letters, 536, 215641.
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4

Multicolor Flow Cytometry Panel

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Cells were incubated on ice with 2 μL of anti-CD16/32 TruStain fcX (BioLegend, San Diego, CA) plus 10 μL of Brilliant Stain Buffer Plus (BD Biosciences, Franklin Lakes, NJ) for 30 min. The cells were incubated with fluorochrome-labeled mAb for 30 min at RT in the dark then were washed twice with 3 mL flow cytometry buffer (PBS + 0.5% BSA + 0.1% NaN3). Cells were post-fixed with 200 μL IC Fixation buffer (Life Technologies Corp.) for 30 min at RT in the dark, then washed again with 3 mL flow cytometry buffer, and stored at 4 °C until analysis. Flow cytometry was performed on a Stratedigm S1200Ex (Stratedigm Inc., San Jose, CA) and data were analyzed with CellCapTure software (Stratedigm).
Cassidy B.R., Sonntag W.E., Leenen P.J, & Drevets D.A. (2022). Systemic Listeria monocytogenes infection in aged mice induces long-term neuroinflammation: the role of miR-155. Immunity & Ageing : I & A, 19, 25.
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