Tragacanth powder

Manufactured by Thermo Fisher Scientific

Tragacanth powder is a natural gum extracted from the sap of various species of the Astragalus plant. It is a water-soluble, high-viscosity polysaccharide that is commonly used as a thickening, stabilizing, and emulsifying agent in a variety of applications, including food, cosmetics, and pharmaceutical formulations.

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Lab products found in correlation

Optimal cutting temperature oct, by Thermo Fisher Scientific (1 mentions) Optimal cutting temperature compound, by Thermo Fisher Scientific (1 mentions)

2 protocols using tragacanth powder

Skeletal Muscle Biopsy and Analysis

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After local anesthesia with lidocaine/bupivacaine, skeletal muscle samples were collected using the Bergstrom technique with suction from the vastus lateralis (Propper Manufacturing Co., Long Island City, NY). Two separate incisions were made to collect tissues at baseline and post-exercise. The second biopsy collections were obtained within a time frame of no more than 3 minutes following the completion of exercise. All skeletal muscle samples were visually assessed and cleared for intramuscular adipose tissue, and then immediately snap frozen in liquid nitrogen for mRNA and Protein measures. Samples were blotted dry and then mounted in a mixture of Optimal Cutting Temperature (OCT, Thermo Scientific, Waltham, MA) and Tragacanth powder (Acros, Geel, Belgium) for immunohistochemical measures of glycogen, intramyocellular lipid, and fiber typing. Another sample was collected for measurements of ex vivo palmitate oxidation.

Covington J.D., Galgani J.E., Moro C., LaGrange J.M., Zhang Z., Rustan A.C., Ravussin E, & Bajpeyi S. (2014). Skeletal Muscle Perilipin 3 and Coatomer Proteins Are Increased following Exercise and Are Associated with Fat Oxidation. PLoS ONE, 9(3), e91675.

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Vastus Lateralis Muscle Biopsy Protocol

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After local anesthesia with 2% lidocaine/0.5% bupivacaine (1/1 ratio), vastus lateralis samples were collected using the Bergstrom technique with suction. Two separate incisions were made to collect tissues at baseline and postexercise. The second biopsy was obtained immediately after the completion of exercise (<3 min). Muscle samples were visually assessed and cleaned of intramuscular adipose tissue. Muscle biopsies were snap frozen in liquid nitrogen for subsequent mRNA and protein analyses, or blotted dry and then mounted in a mixture of optimal cutting temperature compound (Thermo Scientific, Waltham, MA) and tragacanth powder (Acros, Geel, Belgium) for immunohistochemical measures of glycogen, intramyocellular lipid (IMCL), and fiber typing. Another sample was collected for measurements of ex vivo palmitate oxidation.

Covington J.D., Tam C.S., Bajpeyi S., Galgani J.E., Noland R.C., Smith S.R., Redman L.M, & Ravussin E. (2016). Myokine Expression in Muscle and Myotubes in Response to Exercise Stimulation. Medicine and science in sports and exercise, 48(3), 384-390.

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