Wild type C57BL6/N (C57BL/6NTac, 8–10 weeks old) and Swiss-Webster (8–10 weeks) mice were purchased from Taconic. The UBC-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J, 8–13 weeks old; JAX #004353)(Schaefer et al., 2001 (link)) and R26R–YFP (B6.Cg–Gt(ROSA)26Sortm3(CAG−EYFP)Hze/J; JAX #007903) (Madisen et al., 2010 (link)) mice were obtained from the Jackson Laboratory. The Nkx3-1Cre allele has been previously described (Lin et al., 2007 (link); Thomsen et al., 2008 (link)). As hosts for renal grafts, R2G2 mice (B6;129- Rag2tm1FwaIl2rgtm1Rsky/DwIHsd, 8–15 weeks old) were purchased from Envigo, and NOD/SCID mice (NOD.Cg-Prkdcscid/J, 8–14 weeks old; JAX #001303) were purchased from the Jackson Laboratory. To obtain urogenital mesenchyme for tissue recombination and renal grafting, we used E18 Sprague-Dawley rat embryos from timed matings (Charles River #400). Animal studies were approved by and conducted according to standards set by the Columbia University Irving Medical Center (CUIMC) Institutional Animal Care and Use Committee (IACUC).
R26r yfp b6 cg gt rosa 26sortm3 cag eyfp hze j
Manufactured by Jackson ImmunoResearch
The R26R–YFP (B6.Cg–Gt(ROSA)26Sortm3(CAG−EYFP)Hze/J) is a mouse strain that expresses yellow fluorescent protein (YFP) upon Cre-mediated recombination. The YFP reporter is knocked into the Rosa26 locus, a commonly used genomic location for the expression of reporter genes.
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Lab products found in correlation
Ubc gfp c57bl 6 tg ubc gfp 30scha j, by Jackson ImmunoResearch (2 mentions)
R2g2 mice b6 129 rag2tm1fwail2rgtm1rsky dwihsd, by Inotiv (2 mentions)
Nod scid mice nod cg prkdcscid j, by Jackson ImmunoResearch (2 mentions)
Jax 001303, by Jackson ImmunoResearch (2 mentions)
E18 sprague dawley rat embryos, by Charles River Laboratories (2 mentions)
Swiss webster, by Taconic Biosciences (2 mentions)
C57bl 6n c57bl 6ntac, by Taconic Biosciences (1 mentions)
2 protocols using r26r yfp b6 cg gt rosa 26sortm3 cag eyfp hze j
1
Murine Renal Xenograft Protocol
2
Mice Models for Renal Graft Studies
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Wild type C57BL6/N (C57BL6/NTac, 8-10 weeks old) and Swiss-Webster (8-10 weeks) mice were purchased from Taconic. The Ubc-GFP (C57BL/6-Tg(UBC-GFP)30Scha/J, 8-13 weeks old; JAX #004353) (Schaefer et al., 2001) (link) and R26R-YFP (B6.Cg-Gt(ROSA)26Sor tm3(CAG-EYFP)Hze /J; JAX #007903) (Madisen et al., 2010) (link) mice were obtained from the Jackson Laboratory. The Nkx3-1 Cre allele has been previously described (Lin et al., 2007; (link)Thomsen et al., 2008) (link). As hosts for renal grafts, R2G2 mice (B6;129-Rag2 tm1Fwa Il2rg tm1Rsky /DwIHsd, 8-15 weeks old) were purchased from Envigo, and NOD/SCID mice (NOD.Cg-Prkdc scid /J, 8-14 weeks old; JAX #001303) were purchased from the Jackson Laboratory. To obtain urogenital mesenchyme for tissue recombination and renal grafting, we used E18 Sprague-Dawley rat embryos from timed matings (Charles River #400). Animal studies were approved by and conducted according to standards set by the Columbia University Irving Medical Center (CUIMC) Institutional Animal Care and Use Committee (IACUC).
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