Pp1α c 19

The following antibodies were used for Western blotting (WB) and IF: anti–γ-tubulin GTU88 (IF), α-tubulin clone DM1A (IF+WB), α-tubulin clone YL 1/2 (IF), β-actin clone AC15 (IF), GFP (WB), PPP1R13L HPA041231 (WB), myosin 1c HPA 001768 (IF, WB), FLAG M2 (WB) from Sigma-Aldrich; anti-mCherry (WB) and NuMA [EP3976] (WB) from Abcam; anti-iASPP 18590–1-AP (WB) from Proteintech and PCRP-PPP1R13L-2G4 (WB) from Developmental Studies Hybridoma Bank; anti–ERM #3142 (WB), anti-EB1 clone 5 (WB), and anti-HA clone 6E2 (WB) from Cell Signaling Technology; anti-EB1 KT51 (IF), myosin Ic (13; WB), PP1α (C-19; WB) from Santa Cruz Biotechnology; anti-CDK5RAP2, A300-554 (IF) from Bethyl; anti–Phospho-Histone H3 (Ser10) from Millipore (WB); and anti-GFP JL-8 from Clontech (for GFP1-10, WB). GFP-Trap was from Chromotek, anti-HA agarose conjugated beads, tetramethylrhodamine isothiocyanate (TRITC)-phalloidin from Sigma-Aldrich, and Strep-Tactin beads from IBA.