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The SF994 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a core function within the laboratory setting. No further details or interpretations about its intended use can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

C6158, by Merck Group (2 mentions) Bm0003, by Santa Cruz Biotechnology (2 mentions) Sc 204008, by Merck Group (2 mentions) Sml2037, by Thermo Fisher Scientific (2 mentions) Nc1015539, by Thermo Fisher Scientific (2 mentions) Biotek cytation 5 plate reader, by Agilent Technologies (2 mentions) Methyl green, by Vector Laboratories (1 mentions) Rodent block m, by Biocare Medical (1 mentions) Rmr622, by Biocare Medical (1 mentions) Decloaking chamber, by Biocare Medical (1 mentions) Xylene substitute, by Thermo Fisher Scientific (1 mentions) Da vinci green diluent, by Biocare Medical (1 mentions)

3 protocols using sf994

1

Quantifying Colony Formation Assay

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HMC1, HMC3A, and HMC3B cells were seeded in 24-well plates at 800, 600, and 1400 cells/well, respectively. The next day, media was replaced with 500 μL fresh media containing either vehicle (DMSO) or the indicated concentration of drug (BMS-754807 (Sigma-Aldrich #BM0003), PPP (Santa Cruz #SC-204008), SR10221, SR2595 (Sigma-Aldrich #SML2037), or T0070907 (Fisher #NC1015539); final concentration 1% DMSO). Seven days later, media was removed from all wells and cells were fixed in 10% buffered formalin (Fisher #SF994) for 5 min at room temperature. Wells were washed once in ddH2O and then stained in 0.05% crystal violet (Sigma-Aldrich #C6158) for 30 min at room temperature. Wells were then washed an additional 3 times in ddH2O to remove any unbound stain and allowed to dry at room temperature overnight. Once dry, individual wells were imaged at 4X magnification on a BioTek Cytation 5 plate reader (BioTek Instruments, Inc.) and images were stitched using the Gen5 software (v2.09; BioTek Instruments, Inc.) using the default parameters. Colony numbers were quantified manually in ImageJ (Schneider et al., 2012 (link)), where a colony is defined as a cluster of at least 50 individual cells.
Musicant A.M., Parag-Sharma K., Gong W., Sengupta M., Chatterjee A., Henry E.C., Tsai Y.H., Hayward M.C., Sheth S., Betancourt R., Hackman T.G., Padilla R.J., Parker J.S., Giudice J., Flaveny C.A., Hayes D.N, & Amelio A.L. (2021). CRTC1/MAML2 directs a PGC-1α-IGF-1 circuit that confers vulnerability to PPARγ inhibition. Cell reports, 34(8), 108768.
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2

Quantifying Colony Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
HMC1, HMC3A, and HMC3B cells were seeded in 24-well plates at 800, 600, and 1400 cells/well, respectively. The next day, media was replaced with 500 μL fresh media containing either vehicle (DMSO) or the indicated concentration of drug (BMS-754807 (Sigma-Aldrich #BM0003), PPP (Santa Cruz #SC-204008), SR10221, SR2595 (Sigma-Aldrich #SML2037), or T0070907 (Fisher #NC1015539); final concentration 1% DMSO). Seven days later, media was removed from all wells and cells were fixed in 10% buffered formalin (Fisher #SF994) for 5 min at room temperature. Wells were washed once in ddH2O and then stained in 0.05% crystal violet (Sigma-Aldrich #C6158) for 30 min at room temperature. Wells were then washed an additional 3 times in ddH2O to remove any unbound stain and allowed to dry at room temperature overnight. Once dry, individual wells were imaged at 4X magnification on a BioTek Cytation 5 plate reader (BioTek Instruments, Inc.) and images were stitched using the Gen5 software (v2.09; BioTek Instruments, Inc.) using the default parameters. Colony numbers were quantified manually in ImageJ (Schneider et al., 2012 (link)), where a colony is defined as a cluster of at least 50 individual cells.
Musicant A.M., Parag-Sharma K., Gong W., Sengupta M., Chatterjee A., Henry E.C., Tsai Y.H., Hayward M.C., Sheth S., Betancourt R., Hackman T.G., Padilla R.J., Parker J.S., Giudice J., Flaveny C.A., Hayes D.N, & Amelio A.L. (2021). CRTC1/MAML2 directs a PGC-1α-IGF-1 circuit that confers vulnerability to PPARγ inhibition. Cell reports, 34(8), 108768.
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3

Immunohistochemical Analysis of CD34+ Cells

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Tissues were dissected and fixed in 10% neutral buffered formalin (Fisher Scientific, SF99-4) for 24 hours at room temperature and processed in the Hypercenter XP (Thermo Fisher) using a standard dehydration protocol before paraffin embedding and microtome sectioning into 4 μm sections. Tissue sections were deparaffinized with xylene substitute (Thermo Fisher) before rehydration with gradients of alcohol. Heat-induced antigen retrieval was performed in Rodent Decloaker solution (Biocare Medical, RD913) in the Decloaking chamber (Biocare Medical). Non-specific interaction of primary antibodies with the tissue was blocked by Rodent block M (Biocare Medical, RBM961). Tissues were then stained with Rabbit anti-CD34 (Abcam, ab81289) at 1:3000 dilution in Da Vinci Green diluent (Biocare Medical, PD900) overnight at 4° C. The tissue section was stained with Rabbit-on-rodent-HRP polymer (Biocare Medical, RMR622). The slides were treated with Vector ImmPact® VIP peroxidase substrate (Vector labs, SK-4605) for 10 minutes at room temperature. Methyl green (Vector labs, H-3402) was used to counterstain.
Shibad V., Bootwala A., Mao C., Bader H., Vo H., Landesman-Bollag E., Guo C., Rubio A., Near R., Gao W., Challa S., Chukka V., Gao J., Kelly A., Landesman T., VanHelene T, & Zhong X. (2021). L2pB1 Cells Contribute to Tumor Growth Inhibition. Frontiers in Immunology, 12, 722451.
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