The gut microbiota of 8-wk-old C57BL/6 mice was depleted by feeding a mixture of antibiotics in drinking water ad libitum for 4 wk. In brief, mice were provided 1 g/l ampicillin, 500 mg/l vancomycin, 1 g/l neomycin sulfate, and 1 g/l metronidazole in drinking water, as previously described (13 (link)). Bacteroides strains Bacteroides uniformis (ATCC 8492), Bacteroides caccae (ATCC 43185), Parabacteroides goldsteinii (ATCC BAA-1180), Bacteroides ovatus (ATCC 8483), and Bacteroides vulgatus (ATTC 8482) were purchased from American Type Culture Collection (Manassas, VA). Bacteroides strains were cultured in BBL Chopped Meat Carbohydrate Broth, Pre-Reduced II (BD Biosciences, Sparks, MD). Antibiotic-pretreated Itch−/− mice were gavaged 1 × 108 CFU per mouse, and mice were sacrificed after 4 wk.
Bacteroides uniformis
Manufactured by American Type Culture Collection
Bacteroides uniformis is a bacterial strain available from the American Type Culture Collection (ATCC). It is an anaerobic, Gram-negative, rod-shaped bacterium. Bacteroides uniformis is a common inhabitant of the human gut microbiome.
Automatically generated - may contain errors
Lab products found in correlation
Bacteroides caccae, by American Type Culture Collection (2 mentions)
Bacteroides ovatus, by American Type Culture Collection (2 mentions)
Clostridium scindens, by American Type Culture Collection (1 mentions)
Bacteroides vulgatus, by American Type Culture Collection (1 mentions)
Parabacteroides distasonis, by American Type Culture Collection (1 mentions)
2 protocols using bacteroides uniformis
1
Gut Microbiota Depletion and Restoration
2
Culturing Synthetic Gut Microbiome
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Synthetic community strains were cultured in pre-reduced Mega Medium15 (link) or on EG Agar29 at 37 °C in an anaerobic chamber (Coy Laboratories, Grass Lake, MI, USA) with an atmosphere of 5% hydrogen, 10% CO2 and 85% N2. Synthetic community strains included Bacteroides uniformis ATCC 8492, Bacteroides vulgatus ATCC 8482, Bacteroides thetaiotaomicron VPI-5482, Bacteroides caccae ATCC 43185, Bacteroides ovatus ATCC 8483, Parabacteroides distasonis ATCC 8503, Eubacterium rectale ATCC 33656, Dorea longicatena DSM 13814, Ruminococcus torques ATCC 27756, and Clostridium scindens ATCC 35704. All strains were obtained from the American Type Culture Collection (ATCC), except D. longicatena DSM 13814, which was obtained from The Leibniz Institute DSMZ (Braunschweig, Germany).
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