Sirius red, RNAscope and H&E stainings were captured with a Nikon Ti2 Eclipse microscope, equipped with a Nikon Digital Sight D5-VI1 color camera. O-R-O stainings and IHC stainings were taken using a Nikon Eclipse Ci microscope, with a color camera or a S360 Hamamatsu Slidescanner (CECAD Imaging Facility, Cologne). Color brightfield images of HE staining for the spatial transcriptomics experiment were captured with a Nikon Ti2 Eclipse microscope, equipped with a Nikon Digital Sight D5-VI1 color camera and using a 10×/ 0.3 NA CFI Plan-Flour objective. Tilescan image acquisition was carried out using the NIS Elements software.
Nikopoulou C., Kleinenkuhnen N., Parekh S., Sandoval T., Ziegenhain C., Schneider F., Giavalisco P., Donahue K.F., Vesting A.J., Kirchner M., Bozukova M., Vossen C., Altmüller J., Wunderlich T., Sandberg R., Kondylis V., Tresch A, & Tessarz P. (2023). Spatial and single-cell profiling of the metabolome, transcriptome and epigenome of the aging mouse liver. Nature Aging, 3(11), 1430-1445.
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