To evaluate therapeutic effects, the T2DM rats were randomly divided into four groups (n=6 per group). Over a period of 4 weeks, free Ex-4 solution (20 µg/kg, daily) was administered by SC injection, while EL-CS (300 µg/kg, daily) and EL-CSG (300 µg/kg, daily) were administered by gavage at 6 PM, one hour prior to the first meal of the day. After 4 weeks administration, blood samples (200 µL) were collected from the jugular vein, centrifuged (1,800 ×g, 4 °C, 10 min), and the content of Ex-4 (EK-070–94, Phoenix Pharmaceuticals Inc.), hemoglobin A1c (HbA1c, Diazyme Lab., San Diego, CA, USA), total cholesterol (T-Chol, ab65390, Abcam), high-density lipoprotein (HDL, ab65390, Abcam), and low-density lipoprotein (LDL, ab65390, Abcam) in the plasma was quantified using each assay kit.
Ek 070 94
Manufactured by Phoenix Pharmaceuticals
Sourced in United States
The EK-070-94 is a laboratory equipment used for precision liquid handling. It is designed to accurately dispense and aspirate small volumes of liquids in a range of applications within the scientific research and clinical laboratory settings.
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Lab products found in correlation
4 protocols using ek 070 94
1
Evaluating Therapeutic Effects of Ex-4, EL-CS, and EL-CSG in T2DM Rats
2
ELISA for EXE-4 and GLP-1 in Rat Retina
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For the enzyme-linked immunosorbent assay (ELISA) of EXE-4 and GLP-1, pre-weighed (using OHAUS microbalance), whole retina samples (n = 6 for diabetic; and n = 6 for non-diabetic rats) were homogenized in PBS, using a Janke & Kunkel Ultra Turrax t25 homogenizer (Freiburg, Baden-Württemberg, Germany). EXE-4 (Heloderma suspectum) was measured using the ELISA kit EK-070-94 (Phoenix Pharmaceuticals Inc., Burlingame, CA, USA), which had a sensitivity of 0.08 ng/mL (range, 0.08–0.86 ng/mL) and cross reacted only with EXE-4. GLP-1 measurements were performed as per the manufacturer’s protocol (GLP-1—Sigma Aldrich RAB0201). The sensitivity of the assay was 1.17 pg/mL, the intra-assay coefficient of variation (CV) was <10%, and the inter-assay CV was <15%. The reagents did not cross-react with similar cytokines. The plasma of the corresponding rats was used for plasma EXE-4 and GLP-1 ELISA measurements. The measurement of CAT activity was performed using a colorimetric method. The measurement was performed according to the protocol provided with the commercial kit (Cayman Chemical, Ann Arbor, MI, USA).
3
Exendin-4 Quantification from MSC-Derived Exosomes
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Supernatant of MSC-Ex-4 was collected on 12, 24, 48, 60, and 72 hours. Blood samples collected on different time points were obtained from the tail vein and centrifuged at 700g and 4°C for 20 min to collect the serum. The concentration of Exendin-4 in the collected supernatants or serum was then measured using an exenatide ELISA kit (EK-070-94, Phoenix Pharmaceuticals) and calculated on the basis of standard curves for Exendin-4.
4
Exendin-4 Quantification in Exosomes
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The concentration of Exendin-4 in the supernatant and exosomes was quantified 24 h post-construction using an exenatide ELISA kit (EK-070-94, Phoenix Pharmaceuticals), with values calculated against Exendin-4 standard curves. Samples were collected for analysis at three key time points—2, 4, and 6 weeks—during both osteogenic and ECO induction phases.
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