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Cathepsin g activity assay kit colorimetric

Manufactured by Abcam
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Sourced in United Kingdom

The Cathepsin G Activity Assay Kit (Colorimetric) is a laboratory tool used to measure the activity of the enzyme Cathepsin G. It utilizes a colorimetric method to quantify the enzymatic activity.

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Lab products found in correlation

Trypsin activity colorimetric assay kit, by Merck Group (2 mentions) Plasmin activity assay kit fluorometric, by Merck Group (2 mentions)

Close spelling variants of this product

Cathepsin G Activity Assay Kit Cathepsin G Colorimetric assay kit Activity assay kits Colorimetric assay

4 protocols using cathepsin g activity assay kit colorimetric

1

Cathepsin G Activity in Cell and Tissue Assays

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Cells - The cultured medium from 3T3-L1 cells was collected from cells treated with tumor necrosis factor (TNFα, 20 ng/mL) and/or recombinant SERPINA3C protein. Cathepsin G activity was measured using a Colorimetric Cathepsin G Activity Assay kit (Abcam, Cambridge, UK) according to the manufacturer’s protocol.
Mice - Fresh tissues were collected after overnight fasting of the mice. Tissues were lysed in cold PBS and centrifuged at 12000 rpm for 10 min to collect supernatant. Cathepsin G activity was measured using a Colorimetric Cathepsin G Activity Assay kit (Abcam) according to the manufacturer’s protocol.
Li B.Y., Guo Y.Y., Xiao G., Guo L, & Tang Q.Q. (2022). SERPINA3C ameliorates adipose tissue inflammation through the Cathepsin G/Integrin/AKT pathway. Molecular Metabolism, 61, 101500.
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2

Enzymatic Activity Assays

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Plasma CG, trypsin, and plasmin activity were determined using their respective commercially available kits by following the instructions provided with the kits: Cathepsin G Activity Assay Kit (Colorimetric) (ab126780; Abcam, UK), Trypsin Activity Colorimetric Assay Kit (MAK290‐1KT; Sigma, UK), or Plasmin Activity Assay Kit (Fluorometric) (MAK244‐1KT; Sigma, UK).
Wen G., An W., Chen J., Maguire E.M., Chen Q., Yang F., Pearce S.W., Kyriakides M., Zhang L., Ye S., Nourshargh S, & Xiao Q. (2018). Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 7(4), e008187.
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3

Plasma Protease Activity Assays

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Cathepsin G Activity Assay Kit (Colorimetric; ab126780; Abcam), Trypsin Activity Colorimetric Assay Kit (MAK290‐1KT; Sigma, United Kingdom), or Plasmin Activity Assay Kit (Fluorometric; MAK244‐1KT; Sigma, United Kingdom) were used to detect plasma CG (cathepsin G), trypsin, and plasmin activity as per manufacturer’s instructions.
Yang M., Zhou X., Pearce S.W., Yang Z., Chen Q., Niu K., Liu C., Luo J., Li D., Shao Y., Zhang C., Chen D., Wu Q., Cutillas P.R., Zhao L., Xiao Q, & Zhang L. (2023). Causal Role for Neutrophil Elastase in Thoracic Aortic Dissection in Mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 43(10), 1900-1920.
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4

Characterization of Neutrophil Secretome

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Secreted protein samples from purified neutrophils (2 × 107 cells/ml) were examined by ELISA, activity assays and immunoblots. NE levels were determined by ELISA (R&D Systems). Cathepsin G activity was determined in samples with a colorimetric cathepsin G activity assay kit (Abcam, ab126780, Cambridge, MA), as described by manufacturers. Immunoblots were performed with anti-human lactoferrin polyclonal rabbit (immunogen was full length native protein, Cat. # ab15811, Abcam) and anti-human hCAP18 rabbit polyclonal antibodies (Cat. # PA5-20513, Invitrogen). BALF gelatinase activity (MMP9) was determined by gelatin zymography66 (link). Densitometry was performed on MMP9 positive bands and represented densitometry units (DU) measured by pixel intensity of bands, using Bio-Rad Laboratories Image Lab software (version 4.0, build 16). Equal cell numbers in each experiment was demonstrated by profiling electrophoretic of whole cell lysates in a Coomassie blue stained gel, i.e. loading control. O2− production was quantified by O2− dismutase–inhibitable reduction of cytochrome c at 550 nm, as previously described67 (link).
Ochieng P., Nath S., Macarulay R., Eden E., Dabo A., Campos M., Jiang X.C., Foronjy R.F, & Geraghty P. (2018). Phospholipid transfer protein and alpha-1 antitrypsin regulate Hck kinase activity during neutrophil degranulation. Scientific Reports, 8, 15394.
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