RIPA lysis buffer and protease inhibitor cocktail were used for the extraction of total proteins from cells. BCA Protein Assay Kit (Beyotime, China) was used to determine the protein concentrations. Then the protein lysates were separated by SDS-PAGE and transferred to PVDF membranes. After incubation with primary antibodies at 4 °C overnight, the membranes were incubated with the corresponding secondary antibodies at room temperature for 1 h. Finally, the proteins were evaluated by chemiluminescence according to the manufacturer’s instructions. Antibodies used in the present study were: VEGFA (26381-1-AP, Proteintech), β-actin (60008-1-Ig, Proteintech), LAMP1(21997-1-AP, Proteintech). All assays were performed in triplicate.
Vegfa 26381 1 ap
Manufactured by Proteintech
VEGFA (26381-1-AP) is a primary antibody product offered by Proteintech. It is designed to detect the VEGFA (vascular endothelial growth factor A) protein. VEGFA is a key regulator of angiogenesis and plays a crucial role in various physiological and pathological processes.
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Lab products found in correlation
2 protocols using vegfa 26381 1 ap
1
Protein Expression Analysis by Western Blot
2
Protein Extraction and Western Blot
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RIPA lysis buffer and protease inhibitor cocktail were used for the extraction of total proteins from cells. BCA Protein Assay Kit (Beyotime, China) was used to determine the protein concentrations. Then the protein lysates were separated by SDS-PAGE and transferred to PVDF membranes. After incubation with primary antibodies at 4°C overnight, the membranes were incubated with the corresponding secondary antibodies at room temperature for 1h. Finally, the proteins were evaluated by chemiluminescence according to the manufacturer's instructions. Antibodies used in the present study were: VEGFA (26381-1-AP, Proteintech), β-actin (60008-1-Ig, Proteintech). All assays were performed in triplicate.
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