HCC827, MBA-MD-231, and MCF7 cell lines were purchased from the Research Facilities of Peking Union Medical College (PUMC) Cell Bank (Beijing, China). The A549 cell line was purchased from Shanghai Biowing Biotechnology Co (Shanghai, China). A549 and MCF7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Corning, NY, USA) supplied with 10% fetal bovine serum (FBS) (Gemini Bio, CA, USA). HCC827 and MBA-MD-231 cells were maintained in RPMI 1640 medium (Corning, NY, USA) supplied with 10% FBS. 293F cells were grown in Freestyle™ 293 expression medium (OPM, Shanghai, China). All cells were maintained at 37 °C in a humidified chamber containing 5% CO2.
Lentiviral particles were produced to transfect GFP1-10 (#80409, Addgene, Watertown, MA, USA) gene into HCC827 cells according to the protocol of Lentivirus Production (Addgene) [46 (link)]. GFP1-10-positive cells were selected with puromycin (3 μg/mL).
Lentiviral particles were produced to transfect GFP1-10 (#80409, Addgene, Watertown, MA, USA) gene into HCC827 cells according to the protocol of Lentivirus Production (Addgene) [46 (link)]. GFP1-10-positive cells were selected with puromycin (3 μg/mL).