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Goat peroxidase conjugated anti mouse igg secondary anti body

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Goat peroxidase-conjugated anti-mouse IgG secondary antibody is a detection reagent used in immunoassays and Western blotting. It binds to mouse immunoglobulin G (IgG) and is conjugated to the enzyme peroxidase, allowing for colorimetric or chemiluminescent detection of target proteins.

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Lab products found in correlation

Precast polyacrylamide gel, by Bio-Rad (3 mentions) Anti cd63, by Thermo Fisher Scientific (3 mentions) Ripa lysis and extraction buffer, by Thermo Fisher Scientific (3 mentions) Anti calnexin, by Thermo Fisher Scientific (2 mentions) Anti rab27a, by Thermo Fisher Scientific (2 mentions) Anti hcmv capsid mcp, by Bio-Rad (2 mentions) Anti mouse monoclonal antibodies clones, by Bio-Rad (2 mentions) V3 western workflow, by Bio-Rad (2 mentions) Anti p53, by Thermo Fisher Scientific (1 mentions) Anti tsg101, by Thermo Fisher Scientific (1 mentions) Anti hiv 1 p24, by Abcam (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions)

3 protocols using goat peroxidase conjugated anti mouse igg secondary anti body

1

Comparative Proteomic Analysis of Bacterial EV and HIV-1 Proteins

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Total proteins were extracted either from bacterial EVs or from HIV-1-infected/non infected MT-4 cells treated or not treated with bacterial EVs, using RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific, Waltham, MA). We loaded 10 μg of proteins on a 4–20% precast polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA), separated them using SDS-PAGE, and then transferred them to low-fluorescence PVDF membranes and probed them with anti-HIV-1 p24 (0.5 μg per mL; Abcam, Cambridge, MA, ab9071), anti-p53 (1 μg per mL; Thermo Fisher Scientific, Waltham, MA, MA5-12557), anti-CD63 (1 μg per mL; Thermo Fisher Scientific, Waltham, MA, 10628D), anti-TSG101 (1 μg per mL; Thermo Fisher Scientific, Waltham, MA, MA1-23296), primary anti-mouse monoclonal antibodies, and then goat peroxidase-conjugated anti-mouse IgG secondary antibody (Bio-Rad Laboratories, Hercules, CA). We detected peroxidase activity and digital images using the ChemiDoc™ MP Imaging System (Bio-Rad Laboratories, Hercules, CA).
Ñahui Palomino R.A., Vanpouille C., Laghi L., Parolin C., Melikov K., Backlund P., Vitali B, & Margolis L. (2019). Extracellular vesicles from symbiotic vaginal lactobacilli inhibit HIV-1 infection of human tissues. Nature Communications, 10, 5656.
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2

EV and HCMV Protein Characterization

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Total proteins were extracted from both EV and HCMV fractions, according to RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific, Waltham, MA). 10 μg of proteins were loaded on a 4–20% precast polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA) and separated by SDS-PAGE, then transferred to PVDF membranes and probed with anti-CD63 (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Calnexin (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Rab27A (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-HCMV capsid MCP (2 μg/ml), and anti-HCMV tegument pp150 (2 μg/ml) monoclonal primary anti-mouse monoclonal antibodies (clones 28–4 and 36–14) and then goat peroxidase-conjugated anti-mouse IgG secondary anti-body (Bio-Rad Laboratories, Hercules, CA). Peroxidase activity and digital images were detected by using V3 Western Workflow™ (Bio-Rad Laboratories, Hercules, CA).
Zicari S., Arakelyan A., Palomino R.A., Fitzgerald W., Vanpouille C., Lebedeva A., Schmitt A., Bomsel M., Britt W, & Margolis L. (2018). Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins. Virology, 524, 97-105.
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3

EV and HCMV Protein Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total proteins were extracted from both EV and HCMV fractions, according to RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific, Waltham, MA). 10 μg of proteins were loaded on a 4–20% precast polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA) and separated by SDS-PAGE, then transferred to PVDF membranes and probed with anti-CD63 (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Calnexin (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-Rab27A (1 μg/ml, Thermo Fisher Scientific, Waltham, MA), anti-HCMV capsid MCP (2 μg/ml), and anti-HCMV tegument pp150 (2 μg/ml) monoclonal primary anti-mouse monoclonal antibodies (clones 28–4 and 36–14) and then goat peroxidase-conjugated anti-mouse IgG secondary anti-body (Bio-Rad Laboratories, Hercules, CA). Peroxidase activity and digital images were detected by using V3 Western Workflow™ (Bio-Rad Laboratories, Hercules, CA).
Zicari S., Arakelyan A., Palomino R.A., Fitzgerald W., Vanpouille C., Lebedeva A., Schmitt A., Bomsel M., Britt W, & Margolis L. (2018). Human cytomegalovirus-infected cells release extracellular vesicles that carry viral surface proteins. Virology, 524, 97-105.
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