The deparaffinized tissue sections were incubated with mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:800; Millipore, USA), rabbit polyclonal anti-ionized calcium-binding adaptor molecule 1 (Iba-1) antibody (1:1000; Wako, Japan) or rabbit polyclonal anti-cysteine-aspartic acid protease 3 (caspase-3) (1:1000; Cell Science Technology, USA) at 4? overnight [18 (link)]. After washing with PBS, sections were incubated with biotinylated-conjugated goat anti-mouse or rabbit IgG (1:200; Vector Laboratories) for 1 h at 37? and visualized by DAB (Sigma Aldrich, USA).
Biotinylated conjugated goat anti mouse or rabbit igg
Manufactured by Vector Laboratories
Sourced in United States
Biotinylated-conjugated goat anti-mouse or rabbit IgG is a secondary antibody used for detection and quantification purposes in various immunological techniques. It binds to the primary antibody, which is directed against a target antigen, and the biotin moiety allows for further signal amplification or detection.
Automatically generated - may contain errors
2 protocols using biotinylated conjugated goat anti mouse or rabbit igg
1
Immunohistochemical Staining of Neural Markers
2
Immunohistochemical Staining of Neurological Markers
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Immunohistochemical staining was performed as previously described [26 (link)]. In brief, after deparaffinization and hydration, tissue sections were incubated with one of the following primary antibodies: mouse anti-glial fibrillary acidic protein (GFAP; 1:800; Millipore, #AB3594), rabbit anti-ionized calcium-binding adaptor molecule 1 (Iba-1; 1:1000; Wako, #019-19741) and rabbit anti-cysteine-aspartic acid protease 3 (caspase-3; 1:1000; Cell Signaling Technology, #9662) at 4℃ overnight. Following PBS wash, sections were incubated with biotinylated-conjugated goat anti-mouse or rabbit IgG (1:200, Vector Laboratories, #BA-1000-1.5; #BA-9200) for one hour at 37℃ and then visualized with 3-3-diaminobenzidine-4 HCl/H2O2 (DAB, Sigma-Aldrich, #D0426). caspase-3 stained sections were counterstained with eosin.
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