Anti u1a

Antibodies used in this study were anti-SRSF1 (dilution 1:1000, 32–4500, Invitrogen), anti-U1-70K (dilution 1:1000, H111, kindly provided by Dr. Akila Mayeda at Fujita Health University), anti-U1A (dilution 1:1000, PA5-27474, Thermo Fisher Scientific), anti-U1C (dilution 1:1000, SAB4200188, Sigma-Aldrich), anti-His-tag (dilution 1:2000, D291-3, Medical & Biological Laboratories), anti-FLAG (dilution 1:4000, F1804, Sigma), anti-phosphotyrosine (1 µg for each immunoprecipitation assay, 05–321, EMD MILLIPORE), anti-GFP (dilution 1:1000, 11814460001, Roche), and anti-GAPDH (dilution 1:2500, G9545, Sigma-Aldrich).

Antibodies used for immunoblotting were anti-SRSF1 (1:1000, Invitrogen, 32–4500), anti-Rbfox1 (1:100, N-14, Santa Cruz Biotechnology, sc-135476), anti-Rbfox2 (1:2000, Bethyl Laboratories, A300-864A), anti-hnRNP F/H (1:500, Santa Cruz Biotechnology, sc-32310), anti-U1-70K (1:1000, Synaptic Systems, 203011), anti-U1A (1:1000, Thermo Fisher Scientific, PA5-27474), anti-U1C (1:1000, Sigma-Aldrich, SAB4200188), anti-GAPDH (1:2500, Sigma-Aldrich, G9545), anti-6xHis-tag (1:2500, Medical & Biological Laboratories, D291-3), anti-Flag M2 (1:1000, Sigma-Aldrich, F3165), anti-U2AF65 (1:400, MC3, Santa Cruz Biotechnology, sc-53942), anti-GFPT1 (1:500, Abcam, ab125069), anti-RL2 (1:800, Santa Cruz Biotechnology, sc-59624), anti-O-GlcNAc (CTD110.6) (1:2000, Cell Signaling Technology, 9875), anti-β-actin (1:1000, Santa Cruz Biotechnology, sc-47778), and anti-synaptophysin antibodies (1:50, Innovative Research, 18–0130). The secondary antibodies were anti-mouse IgG (1:2000, Cell Signaling Technology, 7076) conjugated to horseradish peroxidase, goat anti-rabbit IgG (1:2000, Cell Signaling Technology, 7074) conjugated to horseradish peroxidase, and Alexa 488-conjugated goat anti-rabbit antibody (1:1000, Thermo Fisher Scientific, A-11034). AChRs were visualized by Alexa 594-conjugated ɑ-bungarotoxin (1:1000, Invitrogen, B-13423).