Rabbit anti β actin a2066

Alirocumab (Sanofi), Evolocumab (Amgen), or non-specific human IgG2 control antibody (BP0301; Bio X Cell) was diluted in 0.9% isotonic saline solution and i.p. applied at a final concentration of 1 µg/g bodyweight. In vitro, blocking of LRP1 function was achieved by using 15 µg/ml mouse anti-11E2 antibody [35 (link)] in comparison to equal amounts of a non-specific mouse IgG control (12-371; Sigma-Aldrich). Human Aβ was immunoprecipitated and detected by mouse anti-IC16 [45 (link)] and the α-chain/full length of LRP1 recognized by mouse anti-11E2 antibody [14 (link)]. Rabbit anti-1704 antibody was used to identify the LRP1 β-chain [46 (link)]. Rabbit anti-LDL receptor antibody 3/43 was kindly provided by Joachim Herz (UT Southwestern, USA). Rabbit anti-PCSK9 (ab125251), rabbit anti-Apolipoprotein E (ApoE) (ab150032), chicken anti-rabbit (ab6831), chicken anti-mouse (ab6706), HRP-conjugated donkey anti-chicken (ab16349), and Alexa488-conjugated goat anti-mouse antibody (ab150117) were purchased from Abcam. Rabbit anti-β-actin (A2066) and HRP-conjugated goat anti-rabbit antibody (A5278) were purchased from Sigma-Aldrich. HRP-conjugated donkey anti-mouse antibody (715–035-151) was purchased from Dianova.

Protein samples were separated on 4–12% Bis-Tris acrylamide gels (Life Technologies) and transferred to PVDF (Millipore). HIF-1α antibody was purchased from Cayman Chemical (10006421) and Novus Bio (NB100-479). Arginase I (H-52) and NOS2 (M19) antibodies were purchased from Santa Cruz Biotechnology. Rabbit anti-β-actin (A2066) was purchased from Sigma. β-tubulin (AA2) was purchased from Millipore. Secondary antibodies were goat anti-rabbit HRP (Thermo), goat anti-mouse HRP (Thermo), donkey anti-mouse IRDye800CW, donkey anti-goat IRDye680RD and donkey anti-rabbit IRDye680RD (Licor). Blots were developed with Supersignal Femto (Thermo) or Amersham ECL (GE Healthcare Life Sciences) or visualized on an Odyssey CLx imager (Licor).