Mz7mel

Manufactured by RIKEN BioResource Center

The MZ7MEL is a laboratory equipment product from RIKEN BioResource Center. It is a compact device designed for melting and preparing samples for various analytical techniques. The core function of the MZ7MEL is to efficiently heat and melt samples to the desired temperature, ensuring consistent and reliable sample preparation.

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Lab products found in correlation

Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Wm115, by American Type Culture Collection (2 mentions) Wm1552c, by American Type Culture Collection (2 mentions) Mmacsf, by Thermo Fisher Scientific (2 mentions) Wm 115, by Thermo Fisher Scientific (2 mentions) Sk mel 28, by Thermo Fisher Scientific (2 mentions) Mmacsf, by RIKEN BioResource Center (2 mentions) Sk mel 28, by American Type Culture Collection (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Hek293t, by American Type Culture Collection (1 mentions) Penicillin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Dulbecco modified eagle medium (dmem), by Corning (1 mentions) Rpmi 1640, by Corning (1 mentions) Colo858, by Corning (1 mentions) Rmpi 1640, by Corning (1 mentions)

2 protocols using mz7mel

Cell Culture Conditions for Melanoma and HEK293T

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Cell lines used in this study were obtained from the Massachusetts General Hospital Cancer Center and originated from the following primary sources: A375, C32, K2, RVH421, WM115, SKMEL28, and WM1552C (ATCC);MMACSF (RIKEN BioResource Center); and MZ7MEL (Johannes Gutenberg University Mainz). HEK293T was from ATCC. C32, K2,MMACSF, SKMEL28, RVH421, and WM115 cell lines were grown in DMEM/F12 (Invitrogen) supplemented with 5% heat inactivated fetal bovine serum (FBS) (Gibco) and 1% sodium pyruvate (Invitrogen). MZ7MEL and WM1552C were grown in RPMI-1640 (Corning) supplemented with 5% FBS and 1% sodium pyruvate (Invitrogen). A375 cells were grown in Dulbecco’s modified eagle medium with 4.5 g/l D-glucose, 4 mM L-glutamine, and 1mM sodium pyruvate (DMEM) (Corning), supplemented with 5% FBS. HEK293T cells were grown in DMEM supplemented with 10% FBS. Penicillin and streptomycin were added to all growth media at final concentrations of 100 U/mL and 100 μg/mL, respectively (Corning). Cells were tested for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza).

Gerosa L., Chidley C., Fröhlich F., Sanchez G., Lim S.K., Muhlich J., Chen J.Y., Vallabhaneni S., Baker G.J., Schapiro D., Atanasova M.I., Chylek L.A., Shi T., Yi L., Nicora C.D., Claas A., Ng T.S., Kohler R.H., Lauffenburger D.A., Weissleder R., Miller M.A., Qian W.J., Wiley H.S, & Sorger P.K. (2020). Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells. Cell systems, 11(5), 478-494.e9.

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Melanoma Cell Line Cultivation Protocol

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Melanoma cell lines used in this study were obtained from the Massachusetts General Hospital Cancer Center with the following primary sources: COLO858 (ECACC), A375, C32, WM115, SKMEL28, and WM1552C (ATCC), LOXIMV1 (DCTD Tumor Repository, National Cancer Institute), MMACSF (RIKEN BioResource Center), and MZ7MEL (Johannes Gutenberg University Mainz). C32, MMACSF, SKMEL28, and WM115 cell lines were grown in DMEM/F12 (Invitrogen) supplemented with 5% fetal bovine serum (FBS) and 1% sodium pyruvate (Invitrogen). COLO858, LOXIMVI, MZ7MEL, and WM1552C cell lines were grown in RMPI 1640 (Corning cellgro) supplemented with 5% FBS and 1% sodium pyruvate (Invitrogen). A375 cells were grown in DMEM with 4.5 g/l glucose, l‐glutamine, and sodium pyruvate (Corning cellgro), supplemented with 5% FBS. We added penicillin (50 U/ml) and streptomycin (50 μg/ml) to all growth media.

Fallahi‐Sichani M., Becker V., Izar B., Baker G.J., Lin J., Boswell S.A., Shah P., Rotem A., Garraway L.A, & Sorger P.K. (2017). Adaptive resistance of melanoma cells to RAF inhibition via reversible induction of a slowly dividing de‐differentiated state. Molecular Systems Biology, 13(1), 905.

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