293T cells and human HCC cell lines Huh7 and Hep3B were purchased from the American Type Culture Collection. Huh7, Hep3B and 293T cells were cultured in Dulbecco's modified Eagles medium (DMEM; HyClone; GE Healthcare Life Sciences) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences), penicillin (100 U/ml) and streptomycin (100 µg/ml) in a humidified atmosphere of 5% CO2 at 37°C. Human umbilical vein endothelial cells (HUVECs) were purchased from PromoCell Academy and cultured in Endothelial Cell Growth Medium 2 (PromoCell Academy) with Supplement Mix according to the manufacturer's guidelines.
Hep3b
Manufactured by GE Healthcare
Sourced in China
Hep3B is a human liver cancer cell line used in laboratory research. It is a commonly used in vitro model for studying liver cancer and related cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hep3b
1
Cell Culture Protocol for Cancer and Endothelial Cells
2
Curative Surgical Resection of HCC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The paired HCC samples and the adjacent non-tumor tissues were collected following curative surgical resection from 48 patients with HCC in the Zhongnan Hospital of Wuhan University between May 2011 and May 2013. This study was approved by the Hospital's Protection of Human Subjects Committee, and written informed consent was obtained from all patients. Based on their medical documents, we conducted a 48-month follow-up survival survey. Overall survival (OS) was defined as the interval between resection and death or the last follow-up visit. Curative resection was defined as the removal of all recognizable tumor tissue with a clear microscopic margin. HepG2, Hep3B, Huh-7, HCCLM9, SK-Hep1, and SMMC-7721 cell lines included in this study were purchased from the Cell Bank of Type Culture Collection (CBTCC, Chinese Academy of Sciences, Shanghai, China) and cultured in Dulbecco's Modified Eagle's Medium (DMEM)/ high-glucose medium (GE Healthcare Life Sciences HyClone Laboratories, Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% penicillin and streptomycin in a humidified atmosphere of 5% CO 2 at 37°C.
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