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Volumescope serial block face em

Manufactured by Thermo Fisher Scientific
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The VolumeScope serial block-face EM is a high-resolution imaging platform designed for three-dimensional analysis of biological samples. It utilizes an electron beam to capture sequential images of a sample's surface, allowing for the reconstruction of a detailed volumetric representation.

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Lab products found in correlation

Vs dbs, by Thermo Fisher Scientific (2 mentions) W1503, by Merck Group (1 mentions) Maps software, by Thermo Fisher Scientific (1 mentions)

2 protocols using volumescope serial block face em

1

Embryo Fixation and Serial Block-Face SEM

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Embryos were fixed in 2.5% glutaraldehyde in embryo grade water (Sigma, W1503) for 1–2 h at room temperature. Fixed embryos were rinsed with 1x PBS and stored at 4 °C before processing. Processing was performed exactly as described previously76 (link). Serial blockface sectioning and scanning electron microscopy were performed using a VolumeScope serial block-face EM (Thermo Fisher, Waltham, MA) equipped with a low-vacuum backscatter detector (VS-DBS; Thermo Fisher).
Hawdon A., Geoghegan N.D., Mohenska M., Elsenhans A., Ferguson C., Polo J.M., Parton R.G, & Zenker J. (2023). Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo. Nature Communications, 14, 2909.
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2

Serial Block-face Electron Microscopy

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Images were additionally collected using a VolumeScope serial block-face EM (SBEM; Thermo Fisher, Waltham, MA) equipped with a low-vac backscatter detector (VS-DBS; Thermo Fisher). Plastic embedded samples were scanned in low vacuum (10 Pa) with a landing beam energy of 2.0 kV and a current of 0.1 nA. Images were acquired using MAPS software (Thermo Fisher, Waltham, MA) at a pixel scale of 5.9–7 nm and pixel dwell of 3 µs.
Rae J., Ferguson C., Ariotti N., Webb R.I., Cheng H.H., Mead J.L., Riches J.D., Hunter D.J., Martel N., Baltos J., Christopoulos A., Bryce N.S., Cagigas M.L., Fonseka S., Sayre M.E., Hardeman E.C., Gunning P.W., Gambin Y., Hall T.E, & Parton R.G. (2021). A robust method for particulate detection of a genetic tag for 3D electron microscopy. eLife, 10, e64630.
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