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3 protocols using octane

1

Synthesis and Characterization of PC Pellets

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PC pellets (2.0 ×
3.0 × 3.0 mm3) were obtained from a local supplier
in Taiwan. All chemicals
were of analytical grade and used without further purification. Acetonitrile
(99.9% w/w), acetone (≥99.5%), heptane (≥99%), and methanol
(anhydrous, 99%) were obtained from Macron. Chloroform (≥99%
w/w) was purchased from Honeywell. While dichloromethane (99%), deuterated
dimethyl sulfoxide (DMSO-d6, 99.9% atom
D), magnesium oxide (97%), 4-nitroaniline (≥99%), sodium aluminate,
sodium methoxide (25wt % in methanol), and sodium chloride were provided
by Sigma-Aldrich, cyclohexane (≥99.0%) was acquired from J.T.
Baker. Alizarin yellow R sodium salt, calcium oxide (99.95%), phenolphthalein,
and strontium oxide (99.5%) were acquired from Alfa Aesar. Bisphenol
A (97%), bromothymol blue, dimethyl carbonate (99%), and tetrahydrofuran
(99.6%) were purchased from Thermo Fisher Scientific. Meanwhile, octane
(>98%) was obtained from Wako.
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2

Solvent Selection and Characterization

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The following 30 solvents (96.0%–99.0% pure) were used without purification: linear alkanes with different carbon chain length (n = 6–16); aromatic hydrocarbons; linear aliphatic alcohols with different carbon chain length (n = 1–9); aromatic alcohols with different carbon chain length of the alkyl group (n = 0–3); acetate esters; unsaturated hydrocarbons; and branched alkanes. Hexane, octane, nonane, decane, dodecane, tetradecane, hexadecane, toluene, styrene, methanol, ethanol, 1-heptanol, 1-octanol, 1,4-butanediol, benzyl alcohol, 2-phenylethanol, propyl acetate, iso -butyl acetate, tert -butyl acetate, butyl acetate, pentyl acetate, octyl acetate, 1-octadecene, and squalene were supplied by Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Butylbenzene, tert-Butylbenzene, octylbenzene, 1-nonanol, and 3-phenyl-1-propanol were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Diluted ethane and ethylene (99.5% pure) and undiluted acetylene (0.1% in N2) were used as standard gases for gas chromatography. These gases were supplied by GL sciences Inc. (Tokyo, Japan). For the spin-trapping agent, we used 5,5-dimethyl-1-pyrroline N-oxide (DMPO; > 97.0% pure), which was supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
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3

DSPE Synthesis and Characterization

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1,2-Di-O-stearoyl-sn-glycero-3-phosphatidylethanolamine (DSPE) was manufactured by Nippon Fine Chemical Co., Ltd. (Osaka, Japan) . Calcium decanoate was purchased from Mitsuwa Chemicals Co., Ltd. (Osaka, Japan) . Calcium stearate, D-glucose, octane, Silica Gel 60, sodium hydroxide, methanol, boron trifluoride methanol complex methanol solution, and n-hexane were purchased from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan) .
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