Mouse embryonic fibroblast cell line NIH‐3T3 was purchased from Cell Bank (Chinese Academy of Sciences, China). Cells were maintained in RPMI‐1640 medium (Invitrogen, Carlsbad, CA) supplemented with 1% penicillin plus streptomycin (HyClone, Logan, UT) and 10% fetal calf serum (Gibco BRL), and then incubated at 37℃ in a humidified incubator (5% CO2). Wild‐type and point mutation of ERBB2 were constructed into plasmids and transfected into cells with Lipofectamine 3000 reagent (Thermo Fisher, USA). After 36 h of transfection, cells were treated with Pyrotinib (200 nm) for additional 48 h. Pyrotinib (SHR‐1258) was purchased from Selleck.
Pyrotinib
Manufactured by Selleck Chemicals
Sourced in United States
Pyrotinib is a chemical compound used in laboratory settings. It is a broad-spectrum kinase inhibitor that targets multiple receptor tyrosine kinases. Pyrotinib is employed in various research applications, including the study of cellular signaling pathways and the development of potential therapeutic agents.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 3000 reagent, by Thermo Fisher Scientific (2 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Poziotinib, by Selleck Chemicals (1 mentions)
Afatinib, by Selleck Chemicals (1 mentions)
Osimertinib, by Selleck Chemicals (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
2 protocols using pyrotinib
1
Pyrotinib Sensitivity in ERBB2 Mutant NIH-3T3 Cells
2
Tarloxotinib and EGFR TKIs Evaluation
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Tarloxotinib and its activated drug, tarloxotinib-E, were provided by Rain Therapeutics Inc. We also used afatinib, poziotinib [second-generation (2G) EGFR TKIs], osimertinib [third-generation (3G) EGFR TKIs], and pyrotinib, which were purchased from Selleck Chemicals (Houston, TX, USA). Each drug was dissolved to a concentration of 10 mmol/L in dimethyl sulfoxide (DMSO, Sigma-Aldrich) and stored at −80 °C until use.
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