2695 separations module system

Manufactured by Waters Corporation

The 2695 Separations Module System is a high-performance liquid chromatography (HPLC) instrument designed for a variety of analytical applications. It offers precise control and monitoring of the chromatographic separation process, including pump, autosampler, and column temperature functionality.

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Lab products found in correlation

High performance liquid chromatography (hplc), by Waters Corporation (1 mentions) Xbridge c18 column, by Waters Corporation (1 mentions) 2996 photodiode array detector, by Waters Corporation (1 mentions)

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2695 separation module (90 citations) 2695 Separations Module (58 citations) 2695 system (35 citations) 2695 Separation module system (2 citations) Waters Alliance 2695 HPLC Separations Module (ELS 2410) System (2 citations) E 2695 Separations Module HPLC system (2 citations)

4 protocols using 2695 separations module system

HPLC Quantification of SDM and 4-ASA

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The concentration of SDM and 4-ASA in solution were determined as previously described ( 18) using an Alliance HPLC with a Waters 2695 Separations module system and Waters 2996 photodiode array detector. The mobile phase consisted of methanol and phosphate buffer pH 6.5 in 40:60 (v/v) ratio. The buffer was prepared from a 50 mM dipotassium phosphate solution adjusted to pH 6.5 with phosphoric acid. The mobile phase was vacuum ® 0.45 µm, 47 mm) and bath sonicated for 5 min. Separation was performed on a Phenomenex Inertsil ODS (3) C18 column ( 150.
An was used. The elution was carried out isocratically at ambient temperature with a flow rate of 1 mL/min. Elution times for 4-ASA and SDM were 1.9 min and 4.0 min respectively. Empower software was used for peak evaluation (Grossjohann et al., 2015) .

Walsh D., Serrano D.R., Worku Z.A., Norris B.A, & Healy A.M. (2018). Production of cocrystals in an excipient matrix by spray drying. International journal of pharmaceutics, 536(1).

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HPLC Quantification of Ibuprofen in Solutions

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The concentration of IBU in solution was determined using an Alliance HPLC with a Waters 2695 Separations module system and Waters 2996 photodiode array detector. The mobile phase consisted of 85% HPLC grade methanol and 15% HPLC grade water containing 0.2% trifluroracetic acid (Li et al., 2016) . The mobile phase was degassed by sonicating for 10 minutes. Separation was performed on a Waters XBridge C18 column with a length of 150
Samples were analysed at a UV detection wavelength of 220 nm. An injection volume of 2 was carried out isocratically with a flow rate of 0.5 mL/min. The temperature of the column chamber was maintained at 40 o C for the entire analytical process. Elution times for IBU and INA were 2.2 and 1.5 minutes respectively. With the systems containing Soluplus, the ratio of aqueous to organic phase was altered to 70:30 v/v due to peak interference. The elution time for IBU was 5.7 minutes. Empower software was used for peak evaluation.

Walsh D., Serrano D.R., Worku Z.A., Madi A.M., O'Connell P., Twamley B, & Healy A.M. (2018). Engineering of pharmaceutical cocrystals in an excipient matrix: Spray drying versus hot melt extrusion. International journal of pharmaceutics, 551(1-2).

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HPLC Quantification of Drug-Loaded Spheres

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HPLC was used to quantify the amount of drug in the spheres. In these experiments, 10 spheres (around 1 mg) were dissolved in 1.5 mL of the mobile phase and analyzed using an Alliance HPLC with a Waters 2695 separations module system and Waters 2996 photodiode array detector. The mobile phase consisted of 40:60 (v/v) phosphate buffer (pH=6.8):acetonitrile for ITR and 40:60 (v/v) water:acetonitrile for POS. The mobile phase was filtered through a 0.45 μm membrane filter (Pall Supor ® 0.45 µm, 47 mm diameter) before use. Separation was performed on a Waters Symmetry ® C18 5 µm (4.6*150 mm) column at a UV detection wavelength of 260 nm with an injection volume of 50 μL. The elution was carried out isocratically at ambient temperature with a flow rate of 1 mL/min. Empower ® software was used for peak evaluation. The calibration curves were linear for both components between 0.05 -50 μg/mL (R 2 > 0.9999).

Mugheirbi N.A., O'Connell P., Serrano D.R., Healy A.M., Taylor L.S, & Tajber L. (2017). A Comparative Study on the Performance of Inert and Functionalized Spheres Coated with Solid Dispersions Made of Two Structurally Related Antifungal Drugs. Molecular pharmaceutics, 14(11).

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HPLC Quantification of SDM and 4-ASA

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The concentration of SDM and 4-ASA was determined using an Alliance HPLC with a Waters 2695 Separations module system and Waters 2996 Photodiode array detector. The mobile phase consisted of methanol/buffer pH 6.5 40/60 (v/v). The buffer was prepared from a 50 mM dipotassium phosphate solution adjusted to pH 6.5 with phosphoric acid. The mobile phase was vacuum filtered through a 0.45 μm membrane filter (Pall Supor ® 0.45 µm, 47 mm) and bath sonicated for 5 min. Separation was performed on a Phenomenex Inertsil ODS (3) C18 column (150 mm length, diameter 4.6 mm, particle size 5 μm) at a UV detection wavelength of 265 nm with an injection volume of 20 μL. The elution was carried out isocratically at ambient temperature with a flow rate of 1 ml/min. The total run time per sample was 6 min. Elution times for 4ASA and SD were 1.9 min and 4.0 min respectively. For peak evaluation Empower software was used. The calibration curves were linear for both components between 100 μg/ml -0.05 μg/ml (R 2 > 0.9999). The linear regression calibration curve obtained for 4ASA was y= 91157x +8454.8 and for SD was y= 80548x + 3498.7.

Serrano D.R., O'Connell P., Paluch K.J., Walsh D, & Healy A.M. (2016). Cocrystal habit engineering to improve drug dissolution and alter derived powder properties. The Journal of pharmacy and pharmacology, 68(5).

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