Caspase activity detection kit

To determine the pathway through which HepG2 cell apoptosis is induced by VPA, the activity of caspases 3, 8 and 9 was measured using the caspase activity detection kit (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China). Briefly, HepG2 cells were induced and collected, and were washed once using PBS. Protein was extracted from the cells using cell lysates contained within the kit, and the activity of caspases 3, 8 and 9 was measured according to the manufacturer’s instructions. A₄₀₅ was used to denote caspase activity.

Human A549 cells were obtained from the Experimental Center of China Medical
University. Fetal bovine serum was purchased from Gemini (USA). The Roswell Park
Memorial Institute (RPMI) 1640 medium was purchased from Hyclone (USA). PQ,
trypsin, MTT and dimethyl sulfoxide (DMSO) were purchased from Sigma (USA).
SP600125, SB203580, ECL luminescent liquid, cell lysate and the bicinchoninic
acid (BCA) quantification kit were purchased from Beyotime Biotechnology
Research Institute (China). The caspase activity detection kit was purchased
from Nanjing Keygen Biotech (China). The annexin V-FITC cell apoptosis detection
kit was purchased from DOJINDO (Japan). The JNK, phospho-JNK(p-JNK), p38 and
phospho-p38(p-P38) antibodies were purchased from Bioworld Technology (USA).