Aperio scanscope cs instrument

Manufactured by Leica

Sourced in United States

The Aperio ScanScope CS instrument is a high-performance, automated digital slide scanner designed for pathology applications. It captures high-resolution images of microscope slides, enabling digital analysis and remote viewing of specimen samples.

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Lab products found in correlation

Imagescope, by Leica (3 mentions) Imagescope software, by Leica (2 mentions) Microvessel analysis v1 algorithm, by Leica (1 mentions)

Close spelling variants of this product

Aperio ScanScope (168 citations) Aperio ScanScope CS (71 citations) ScanScope CS (46 citations) ScanScope®CS instrument (2 citations)

5 protocols using aperio scanscope cs instrument

Immune score evaluation in tumour

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To evaluate immune score in the tumour centre and invasive front, the whole tumour section was necessary, and 311 cases were available for the analysis. Immune score was evaluated by image analysis for CD3- and CD8-expressing lymphocytes in the tumour centre and invasive front, as described previously.^{21 (link)} Briefly, immunohistochemical stain was performed by using anti-CD3 (Ready-to-use, Ventana, 2GV6) and anti-CD8 (Ready-to-use, Ventana, SP57) antibodies, and the immunostained slides were scanned on an Aperio ScanScope CS instrument (Aperio Technologies, Inc.). CD3 + and CD8 + lymphocytes were automatically counted by the Nuclear v9 algorithm of ImageScope^TM (Aperio Technologies, Inc.) image analysis system. We estimated four densities (number of positive cells per mm²) of CD3 + and CD8 + lymphocytes in the tumour centre and invasive front, respectively. If the density of both CD3⁺ and CD8⁺ T cells was elevated (higher than median) in both the centre and front, a high immune score was given.

Park J.W., Chang H.J., Yeo H.Y., Han N., Kim B.C., Kong S.Y., Kim J, & Oh J.H. (2020). The relationships between systemic cytokine profiles and inflammatory markers in colorectal cancer and the prognostic significance of these parameters. British Journal of Cancer, 123(4), 610-618.

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Quantitative Scoring of Alpha-Synuclein Pathology

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All IHC sections were digitally scanned using an Aperio ScanScope CS instrument (40 × magnification; Aperio Technologies Inc., Vista, CA, USA), and images of representative areas of pathology were captured using the ImageScope software (40 × magnification; Aperio Technologies Inc. Vista, CA, USA). Tissue sections were manually scored for αSyn pathology on a scale of 0 (no pathology) to 3 (highest pathology) by three independent raters using the Allen Brain Atlas to define regions (Allen Reference Atlas—Mouse Brain [brain atlas]. Available from atlas.brain-map.org) [1 , 8 (link), 19 (link), 26 (link), 33 (link)]. Morphological descriptions were classified using categories defined previously [29 (link)]. Representative images were corrected for color/hue values; brightness/contrast adjustments were applied identically on captured images within each figure using Adobe Photoshop CS3 (Adobe Systems, San Jose, CA, USA). All raw files are available upon request.

Lloyd G.M., Long B., Quintin S., Sorrentino Z.A., Gorion K.M., Bell B.M., Carrillo D., Sullivan P., Borchelt D, & Giasson B.I. (2023). Carboxyl truncation of α-synuclein occurs early and is influenced by human APOE genotype in transgenic mouse models of α-synuclein pathogenesis. Acta Neuropathologica Communications, 11, 119.

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Quantitative Immunohistochemical Analysis

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All IHC sections were digitally scanned using an Aperio ScanScope CS instrument (40× magnification; Aperio Technologies Inc., Vista, CA, USA), and images of representative areas of pathology were captured using the ImageScope software (40× magnification; Aperio Technologies Inc.). Tissue sections were analyzed using Aperio ImageScope. Regions of interest (ROIs) were selected and quantified separately using a modified version of ImageScope’s Color Deconvolution algorithm v9, tailored to each staining, and slides were scored based on the quantified optical density (OD) analysis of the immunoreactive area (IRA) for the DAB color channel. For analysis, scores were normalized by setting the highest score from all cohorts as the maximum value. Representative images were adjusted for white values; brightness/contrast corrections were applied identically on captured images within each figure using Adobe Photoshop CS3 (Adobe Systems, San Jose, CA, USA). All raw files and algorithms are available upon request.

Lloyd G.M., Sorrentino Z.A., Quintin S., Gorion K.M., Bell B.M., Paterno G., Long B., Prokop S, & Giasson B.I. (2022). Unique Seeding Profiles and Prion-like Propagation of Synucleinopathies are Highly Dependent on the Host in Human α-Synuclein Transgenic Mice. Acta neuropathologica, 143(6), 663-685.

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Digitized Immunohistochemistry Imaging Protocol

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All slides were digitally scanned using an Aperio ScanScope CS instrument (40× magnification; Aperio Technologies Inc., Vista, CA), and images of antibody staining were captured using the ImageScope software (40× magnification; Aperio Technologies Inc). Immunoblots and IHC images were individually white leveled. Brightness and contrast corrections were applied to each IHC figure using Adobe Photoshop (Adobe Systems, San Jose,CA,USA). Raw files are available upon request.

Paterno G., Bell B.M., Gorion K.M., Prokop S, & Giasson B.I. (2022). Reassessment of Neuronal Tau Distribution in Adult Human Brain and Implications for Tau Pathobiology. Acta Neuropathologica Communications, 10, 94.

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Automated Quantification of Tumor Angiogenesis

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Slides were concurrently evaluated by two pathologists (H.E.L and H.S.L) using light microscopy to improve the accuracy of the results (Fig. 1). CRC cells were considered as internal negative controls. Medium- to large-sized vessels were considered as internal positive controls for CD31 and D2-40. Intestinal muscular layer or medium- to large-sized vessels were considered as internal positive controls for desmin and SMA. Samples showing inappropriate staining in internal negative or positive controls were considered non-informative and were excluded from the analysis. Slides were scanned using an Aperio ScanScope® CS instrument (Aperio Technologies, Inc., Vista, CA) at 20× magnification. Subsequently, they were analyzed in ImageScope™ using the Microvessel Analysis v1 algorithm (Aperio Technologies), and MVD and LVD were calculated. Because desmin-positive muscularis mucosa and propria are positive for SMA immunostaining, the area of CAFs (mm²) was calculated by subtracting the areas of desmin staining from that of SMA staining (SMA - desmin).

Kwak Y., Lee H.E., Kim W.H., Kim D.W., Kang S.B, & Lee H.S. (2014). The Clinical Implication of Cancer-Associated Microvasculature and Fibroblast in Advanced Colorectal Cancer Patients with Synchronous or Metachronous Metastases. PLoS ONE, 9(3), e91811.

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