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Izon control suite software

Manufactured by Izon Science
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Sourced in United Kingdom

Izon Control Suite is a software application developed by Izon Science. It provides a user interface and data analysis tools for Izon's range of laboratory equipment. The software enables users to operate the instruments, acquire data, and analyze the results.

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Lab products found in correlation

Izon control suite, by Izon Science (2 mentions) Qnano platform, by Izon Science (1 mentions) Cpc200, by Izon Science (1 mentions) Np200, by Izon Science (1 mentions) Exoid, by Izon Science (1 mentions) Qnano, by Izon Science (1 mentions)

5 protocols using izon control suite software

1

Exosome Size Distribution Analysis

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The size distribution and concentration of Exos were measured by TRPS analysis using a qNano platform with an NP100-rated nanopore (Izon Science, UK). Exosomal samples were diluted 1000-fold with PBS and measured three times. Data processing and analysis were carried out on the Izon Control Suite software (Izon Science, UK).
Biagioni A., Laurenzana A., Menicacci B., Peppicelli S., Andreucci E., Bianchini F., Guasti D., Paoli P., Serratì S., Mocali A., Calorini L., Del Rosso M., Fibbi G., Chillà A, & Margheri F. (2020). uPAR-expressing melanoma exosomes promote angiogenesis by VE-Cadherin, EGFR and uPAR overexpression and rise of ERK1,2 signaling in endothelial cells. Cellular and Molecular Life Sciences, 78(6), 3057-3072.
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2

Characterizing Extracellular Vesicle Size

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Quantification and size characterization of EVs were measured using a tunable resistive pulse sensing (TRPS) instrument (Exoid; IZON Science Ltd, Christchurch, New Zealand). Two different nanopores (NP250, NP400, IZON Science Ltd.) were used to assessment in the size range. Carboxylate polystyrene calibration particles (CPC200 and CPC 500, IZON Science Ltd.) were used with NP200 and NP400 nanopores to ensure optimization conditions (e.g., size, concentration). All calibrations and sample measurements were run under the same conditions recommended by the manufacturer and a minimum of 500 particles was recorded at three different pressures. The acquired data was analyzed using Izon Control Suite software (Izon Control Suite version 3.2.2.268, Izon Science Ltd.).
Kang S.G., Choi Y.Y., Mo S.J., Kim T.H., Ha J.H., Hong D.K., Lee H., Park S.D., Shim J.J., Lee J.L, & Chung B.G. (2023). Effect of gut microbiome-derived metabolites and extracellular vesicles on hepatocyte functions in a gut-liver axis chip. Nano Convergence, 10, 5.
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3

Exosome Size and Density Characterization

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The size and particle density of the exosomes were measured by TRPS (Tunable Resistive Pulse Sensing, Izon Science Ltd, Christchurch, New Zealand) according to the manufacturer’s instructions. The density of the exosome preparation was normalized using 1×1013 particles/ml calibration beads (18 (link)). The data was analyzed using Izon Control Suite software v.3.3.2.2000 (Izon Control Suite version 3.3.2.2001; Izon Science).
Zhang Z., Tang Y., Song X., Xie L., Zhao S, & Song X. (2020). Tumor-Derived Exosomal miRNAs as Diagnostic Biomarkers in Non-Small Cell Lung Cancer. Frontiers in Oncology, 10, 560025.
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4

Exosome Size and Concentration Analysis

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The size of the vesicles and the particle concentration of exosomes were measured by using TRPS (qNano; Izon Science Ltd.). The tunable nanopores with proprietary data capture and analysis using Izon Control Suite software v.3.3.2.2000 (Izon Science Ltd.).
Zhao Y., Song X., Song X, & Xie L. (2022). Identification of Diagnostic Exosomal LncRNA-miRNA-mRNA Biomarkers in Colorectal Cancer Based on the ceRNA Network. Pathology and Oncology Research, 28, 1610493.
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5

Nanoparticle size analysis using TRPS

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MVs were isolated from mLN‐ and pLN‐iFRCs as described above, followed by TRPS analysis using qNano instrument (IZON Sciences Ltd.) as described previously 62. Carboxylated polystyrene particle standards were used to determine the vesicle size. Main characteristics of calibration particles and TRPS measurement are summarized in Supporting Information Table 2. Dilution of samples varied between 10 and 40 times. We counted at least 500 events/sample. Izon Control Suite software (v3.1, Izon Science) enabled to convert number and magnitude of measured pore blockades to particle diameter and concentration.
Pasztoi M., Pezoldt J., Beckstette M., Lipps C., Wirth D., Rohde M., Paloczi K., Buzas E.I, & Huehn J. (2017). Mesenteric lymph node stromal cell‐derived extracellular vesicles contribute to peripheral de novo induction of Foxp3+ regulatory T cells. European Journal of Immunology, 47(12), 2142-2152.
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