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Afm 1

Manufactured by Romer Labs
Sourced in United States

The AFM 1 is a laboratory instrument designed for atomic force microscopy (AFM) analysis. It provides high-resolution imaging and measurement of surface topography at the nanoscale level.

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2 protocols using afm 1

1

Simultaneous Mycotoxin Quantification Protocol

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Methanol (MeOH; LC gradient grade), acetonitrile (ACN; LC gradient grade), and glacial acetic acid (HAc; MS grade) were purchased from Merck (Darmstadt, Germany). Mycotoxin standards were purchased from Romer Labs Diagnostic GmbH Tulln, Austria, including: nivalenol (NIV), 13 C-NIV, DON, 13 C-DON, deepoxy-DON (DOM-1), OTA, 13 C-OTA, AFM 1 , 13 C-AFM 1 , citrinin (CIT), FB 1 , 13 C-FB 1 , 13 C-ZEN or Sigma, Vienna, Austria (ZEN, α-and β-zearalenol (ZEL)). The deuterated [ 2 H 4 ] alternariol (AOH) was synthesized by [31] (link) and kindly provided by Prof. Michael Rychlik, TU Munich. Solid standard substances were dissolved in pure ACN (α-and β-ZEL; [ 2 H 4 ] AOH). All other standards were delivered in either ACN or ACN/H 2 O (FB 1 ) and stored at -20 °C. A combined multi-standard working solution for preparation of calibrants and spiking experiments was prepared in ACN, and also, fresh mixture of 13 C and deuterated [ 2 H 4 ] stable isotope standards were prepared as described by [32] .
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2

Quantification of Aflatoxin Levels

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Methanol, acetone, and acetonitrile (all HPLC grade) were purchased from Merck (Darmstadt, Germany). Ammonium acetate (HPLC grade) was obtained from Sigma-Aldrich (St. Louis, MO, United States). Water was filtered using a Millipore system (Millipore, Billerica, MA, United States). AFB1 (2.03 μg ml−1), and AFM1 (0.5 μg ml−1) of analytical standard were purchased from Romer Labs (Union, MO, United States).
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