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2 protocols using h3k14ac

1

Characterization of Histone Acetylation Markers

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Rabbit polyclonal anti-Sirt6 (Cat#: S4197), mouse monoclonal anti-tubulin-FITC (Cat#:T6074) antibody were purchased from Sigma. Human anti-centromere CREST antibody (Cat#:15–234) was purchased from Antibodies Incorporated (Davis, CA, USA). Cy5-conjugated donkey anti-human IgG (Cat#: 709-605-149) was purchased from Jackson ImmunoResearch Laboratory (West Grove, PA, USA). FITC-conjugated goat anti-rabbit IgG was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Rabbit monoclonal H4K16ac (Cat#: ab109463), rabbit monoclonal H3K56ac (Cat#: ab76307) antibodies were purchased from Abcam (Cambridge, MA, USA). Rabbit polyclonal H3K9ac (Cat#: 9671) and H4K12ac (Cat#: 06–1352) antibodies were purchased from EMD Millipore (MA, USA). And rabbit polyclonal H3K14ac (Cat#: A-4023-050) antibody was purchased from Epigentek Group Inc (Brooklyn, NY, USA).
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2

Histone Modulations in Psychostimulant Abuse

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Psychostimulants–cocaine and METH, Opioid- morphine and piracetam (purity > 99%) were purchased from Sigma-Aldrich (CAS- St. Louis, MO, USA). Cell culture reagents were purchased from ScienCell (Carlsbad, CA, USA). Primary antibodies against HDACs (1–7) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). The HATs PCAF and GCN5 were purchased from Proteintech (Rosemont, IL) and p300 was purchased from Epigentek (Farmingdale, NY). Histone H3 Acetylation Antibody Panel Pack I (H3K9ac, H3K14ac, H3K18ac, H3K27ac) and H3K56ac were purchased from Epigentek (Farmingdale, NY, USA). A polyclonal histone-H3 antibody was purchased from Proteintech (Rosemont, IL). Electrophoresis reagents and nitrocellulose membranes were purchased from Bio-Rad (Richmond, CA, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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