Five milliliters of slurry were mixed with 1 mL of 25% meta-phosphoric acid solution (Sigma-Aldrich, St. Louis, MO, USA) and 0.05 mL of saturated mercury (II) chloride solution (Sigma-Aldrich, St. Louis, MO, USA) in a 15 mL plastic tube. The mixed solution was then centrifuged at 3,134 × ɡ for 20 min at 20°C. One milliliter of supernatant was subsequently centrifuged for 10 min at 13,800 × ɡ and filtered through a 0.2 μm filter (Whatman, Uppsala, Sweden). Filtrates were transferred to 2.0 mL GC vials (Agilent, Santa Clara, CA, USA). The concentration of VFAs was analyzed using a GC (6890N, Agilent, Santa Clara, CA, USA) equipped with a HP-INNOWax column (30 m × 0.25 mm × 0.25 μm; Agilent, Santa Clara, CA, USA) and a flame ionization detector (FID). The sample injection volume was 0.2 μL with a 10:1 split ratio. The oven temperature was initially temperature of 80°C for 2 min, increasing to 120°C at 20°C/min, then to 205°C at 10°C/min, and finally held at 205°C for 2 min. The injection and detection ports were maintained at 250°C.
2.0 ml gc vial
Manufactured by Agilent Technologies
Sourced in United States
The 2.0 mL GC vials are sample containers designed for use in gas chromatography (GC) analysis. They provide a standard 2.0 mL capacity to hold liquid or gaseous samples for injection into a GC system. The vials are made of inert materials to ensure sample integrity during analysis.
Automatically generated - may contain errors
Lab products found in correlation
Hp innowax column, by Agilent Technologies (2 mentions)
0.2 μm filter, by Cytiva (2 mentions)
Whatman, by Cytiva (2 mentions)
Meta phosphoric acid solution, by Merck Group (1 mentions)
Saturated mercury 2 chloride solution, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Db 1 column, by Agilent Technologies (1 mentions)
Chloroform, by Agilent Technologies (1 mentions)
4 m sodium hydroxide solution, by Merck Group (1 mentions)
4 protocols using 2.0 ml gc vial
1
Volatile Fatty Acid Analysis from Slurry
2
Quantitative GC-MS Analysis of Volatile Compounds
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4 g of the
homogenized berry sample or 4 mL of wine was transferred into 20 mL
GC vials purchased from Agilent. 16 μL of ethanolic d3-guaiacol
(5 mg/L) internal standard was added to the samples (final concentration
of 20 μg/kg in berry homogenate or 20 μg/L in wine). Glycosidase
enzymes were then added to the samples. For enzymatic hydrolysis of
real-world samples, the final concentrations of 4 and 1 mg/mL of CbGglB-1 and AoryRut were added, respectively.
The reactions were conducted at 37 °C for 4 h. Forty percent
w/v of NaCl was then added to the samples to stop the reactions, and
GC vials were capped for GC–MS analysis.
homogenized berry sample or 4 mL of wine was transferred into 20 mL
GC vials purchased from Agilent. 16 μL of ethanolic d3-guaiacol
(5 mg/L) internal standard was added to the samples (final concentration
of 20 μg/kg in berry homogenate or 20 μg/L in wine). Glycosidase
enzymes were then added to the samples. For enzymatic hydrolysis of
real-world samples, the final concentrations of 4 and 1 mg/mL of CbGglB-1 and AoryRut were added, respectively.
The reactions were conducted at 37 °C for 4 h. Forty percent
w/v of NaCl was then added to the samples to stop the reactions, and
GC vials were capped for GC–MS analysis.
3
Volatile Fatty Acid Quantification
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Five milliliters of slurry sample was mixed with 1 mL of 25% meta-phosphoric acid (Sigma-aldrich, St. Louis, MO, USA) solution and 0.05 mL of saturated mercury (II) chloride (Sigma-aldrich, USA) solution in a 15 mL plastic tube. The mixed solution was then centrifuged at 4,000 rpm for 20 min at 20°C, and 1 mL of supernatant was transferred to 1.5 mL tube. The supernatant was subsequently centrifuged for 10 min at 12,000 rpm and filtered through a 0.2 μm filter (Whatman, Uppsala, Sweden). Filtrates were transferred to 2.0 mL GC vial (Agilent, Santa Clara, CA, USA). Concentration of VFA was analyzed using a GC (6890N, Agilent, USA) equipped with HP-INNOWax column (30 m×0.25 mm×0.25 μm, Agilent, USA) and flame ionization detector (FID). The oven temperature was programmed to initial temperature of 80°C for 2 min, increasing to 120°C at a rate of 20°C/min, increasing to 205°C at a rate of 10°C/min, and holding at 205°C for 2 min. The injection and detection ports were maintained at 250°C. The sample injection volume was 0.2 μL with a 10:1 split ratio.
4
Extraction and Analysis of Phenols and Indoles
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Slurry samples were centrifuged at 3,134 × ɡ for 20 min at 20°C, and then 4 mL of supernatant was mixed with 4 mL of chloroform (Merck, Darmstadt, Germany) and 60 μL of 4M sodium hydroxide solution (Sigma-Aldrich, St. Louis, MO, USA) in a 20 mL glass vial. The mixture was centrifuged at 3,134 × ɡ for 20 min at 20°C, and the chloroform layer was transferred to a 2.0 mL GC vial (Agilent, Santa Clara, CA, USA). Phenols and indoles were analyzed using a GC (6890N, Agilent, Santa Clara, CA, USA) equipped with a DB-1 column (30 m × 0.25 mm × 0.25 μm, Agilent, Santa Clara, CA, USA) and a FID. The sample injection volume was 2.0 μL with a 5:1 split ratio. The oven temperature was initially 40°C for 5 min, increasing to 230°C at 10°C/min, which was then held at 230°C for 2 min. The injection and detection ports were maintained at 250°C.
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