Supragradient acetonitrile acn

Manufactured by Biosolve

Sourced in Netherlands

SupraGradient acetonitrile (ACN) is a high-purity solvent used in various laboratory applications. It is a colorless, volatile liquid with a characteristic odor. Acetonitrile is commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) and other analytical techniques.

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Lab products found in correlation

Trifluoroacetic acid, by Merck Group (2 mentions) Ammonium bicarbonate, by Merck Group (2 mentions) Formic acid, by Merck Group (2 mentions)

Spelling variants of this product

Acetonitrile (171 citations) Acetonitrile (ACN) (38 citations) HPLC SupraGradient acetonitrile (ACN) (6 citations)

2 protocols using supragradient acetonitrile acn

Phosphate Buffer Preparation Protocol

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Disodium hydrogen phosphate dihydrate (Na₂HPO₄⋅2H₂O), potassium dihydrogen phosphate (KH₂PO₄), NaCl, and trifluoroacetic acid were purchased from Merck (Darmstadt, Germany). Formic acid, ammonium bicarbonate, and TPCK (tosylsulfonyl phenylalanyl chloromethyl ketone)-treated trypsin from bovine pancreas were obtained from Sigma-Aldrich (Steinheim, Germany). Furthermore, high-performance liquid chromatography (HPLC) SupraGradient acetonitrile (ACN) was obtained from Biosolve (Valkenswaard, The Netherlands), and ultrapure deionized water (MQ) was generated by the Purelab Ultra, maintained at 18.2 MΩ (Veolia Water Technologies Netherlands BV, Ede, The Netherlands). Phosphate-buffered saline (PBS [pH 7.3]) was made in house, containing 5.7 g/liter Na₂HPO₄⋅2H₂O, 0.5 g/liter KH₂PO₄, and 8.5 g/liter NaCl.

de Haan N., Boeddha N.P., Ekinci E., Reiding K.R., Emonts M., Hazelzet J.A., Wuhrer M, & Driessen G.J. (2018). Differences in IgG Fc Glycosylation Are Associated with Outcome of Pediatric Meningococcal Sepsis. mBio, 9(3), e00546-18.

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Optimized Peptide Sample Preparation

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Formic acid, ammonium bicarbonate, and TPCK (tosylsulfonyl phenylalanyl chloromethyl ketone)-treated trypsin from bovine pancreas were obtained from Sigma-Aldrich (Steinheim, Germany). SupraGradient acetonitrile (ACN) was obtained from Biosolve (Valkenswaard, Netherlands), and ultrapure deionized water (MQ) was generated by Purelab Ultra, maintained at 18.2 M (Veolia Water Technologies Netherlands BV, Ede, Netherlands). Trifluoroacetic acid was purchased from Merck (Darmstadt, Germany).

Chinello C., de Haan N., Capitoli G., Trezzi B., Radice A., Pagani L., Criscuolo L., Signorini S., Galimberti S., Sinico R.A., Wuhrer M, & Magni F. (2022). Definition of IgG Subclass-Specific Glycopatterns in Idiopathic Membranous Nephropathy: Aberrant IgG Glycoforms in Blood. International Journal of Molecular Sciences, 23(9), 4664.

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